BRENDA - Enzyme Database show
show all sequences of 2.9.1.2

RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea

Yuan, J.; Palioura, S.; Salazar, J.C.; Su, D.; O'Donoghue, P.; Hohn, M.J.; Cardoso, A.M.; Whitman, W.B.; Söll, D.; Proc. Natl. Acad. Sci. USA 103, 18923-18927 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Homo sapiens
-
Methanocaldococcus jannaschii
-
Methanococcus maripaludis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O-phospho-L-seryl-tRNASec + selenophosphate
Methanococcus maripaludis
L-phosphoseryl-tRNA is the crucial precursor for L-selenocysteinyl-tRNA formation in archaea and eukarya. Selenocysteine formation is achieved by a two-step process: O-phosphoseryl-tRNASec kinase (PSTK) phosphorylates the endogenous Ser-tRNASec to O-phosphoseryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase (SepSecS)
L-selenocysteinyl-tRNASec + phosphate
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
Methanocaldococcus jannaschii
L-phosphoseryl-tRNA is the crucial precursor for L-selenocysteinyl-tRNA formation in archaea and eukarya. Selenocysteine formation is achieved by a two-step process: O-phosphoseryl-tRNASec kinase (PSTK) phosphorylates the endogenous Ser-tRNASec to O-phosphoseryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase (SepSecS)
L-selenocysteinyl-tRNASec + phosphate
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
Homo sapiens
L-phosphoseryl-tRNA is the crucial precursor for L-selenocysteinyl-tRNA formation in archaea and eukarya. Selenocysteine formation is achieved by a two-step process: O-phosphoseryl-tRNASec kinase phosphorylates the endogenous L-seryl-tRNASec to O-phospho-L-seryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase
L-selenocysteinyl-tRNASec + phosphate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
Q9HD40
-
-
Methanocaldococcus jannaschii
Q58027
-
-
Methanococcus maripaludis
Q6LZM9
-
-
Purification (Commentary)
Commentary
Organism
recombinant enzyme
Homo sapiens
recombinant enzyme
Methanocaldococcus jannaschii
recombinant enzyme
Methanococcus maripaludis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
O-phospho-L-seryl-tRNASec + selenophosphate
-
694847
Homo sapiens
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
-
694847
Methanococcus maripaludis
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
-
694847
Methanocaldococcus jannaschii
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
L-phosphoseryl-tRNA is the crucial precursor for L-selenocysteinyl-tRNA formation in archaea and eukarya. Selenocysteine formation is achieved by a two-step process: O-phosphoseryl-tRNASec kinase (PSTK) phosphorylates the endogenous Ser-tRNASec to O-phosphoseryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase (SepSecS)
694847
Methanococcus maripaludis
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
L-phosphoseryl-tRNA is the crucial precursor for L-selenocysteinyl-tRNA formation in archaea and eukarya. Selenocysteine formation is achieved by a two-step process: O-phosphoseryl-tRNASec kinase (PSTK) phosphorylates the endogenous Ser-tRNASec to O-phosphoseryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase (SepSecS)
694847
Methanocaldococcus jannaschii
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
L-phosphoseryl-tRNA is the crucial precursor for L-selenocysteinyl-tRNA formation in archaea and eukarya. Selenocysteine formation is achieved by a two-step process: O-phosphoseryl-tRNASec kinase phosphorylates the endogenous L-seryl-tRNASec to O-phospho-L-seryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase
694847
Homo sapiens
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
the enzyme needs pyridoxal 5'-phosphate to carry out the conversion of O-phosphoserine to selenocysteine
Homo sapiens
pyridoxal 5'-phosphate
the enzyme needs pyridoxal 5'-phosphate to carry out the conversion of O-phosphoserine to selenocysteine
Methanocaldococcus jannaschii
pyridoxal 5'-phosphate
the enzyme needs pyridoxal 5'-phosphate to carry out the conversion of O-phosphoserine to selenocysteine
Methanococcus maripaludis
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
-
Methanocaldococcus jannaschii
-
Methanococcus maripaludis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
pyridoxal 5'-phosphate
the enzyme needs pyridoxal 5'-phosphate to carry out the conversion of O-phosphoserine to selenocysteine
Homo sapiens
pyridoxal 5'-phosphate
the enzyme needs pyridoxal 5'-phosphate to carry out the conversion of O-phosphoserine to selenocysteine
Methanocaldococcus jannaschii
pyridoxal 5'-phosphate
the enzyme needs pyridoxal 5'-phosphate to carry out the conversion of O-phosphoserine to selenocysteine
Methanococcus maripaludis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
O-phospho-L-seryl-tRNASec + selenophosphate
Methanococcus maripaludis
L-phosphoseryl-tRNA is the crucial precursor for L-selenocysteinyl-tRNA formation in archaea and eukarya. Selenocysteine formation is achieved by a two-step process: O-phosphoseryl-tRNASec kinase (PSTK) phosphorylates the endogenous Ser-tRNASec to O-phosphoseryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase (SepSecS)
L-selenocysteinyl-tRNASec + phosphate
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
Methanocaldococcus jannaschii
L-phosphoseryl-tRNA is the crucial precursor for L-selenocysteinyl-tRNA formation in archaea and eukarya. Selenocysteine formation is achieved by a two-step process: O-phosphoseryl-tRNASec kinase (PSTK) phosphorylates the endogenous Ser-tRNASec to O-phosphoseryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase (SepSecS)
L-selenocysteinyl-tRNASec + phosphate
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
Homo sapiens
L-phosphoseryl-tRNA is the crucial precursor for L-selenocysteinyl-tRNA formation in archaea and eukarya. Selenocysteine formation is achieved by a two-step process: O-phosphoseryl-tRNASec kinase phosphorylates the endogenous L-seryl-tRNASec to O-phospho-L-seryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase
L-selenocysteinyl-tRNASec + phosphate
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant enzyme
Homo sapiens
recombinant enzyme
Methanocaldococcus jannaschii
recombinant enzyme
Methanococcus maripaludis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
O-phospho-L-seryl-tRNASec + selenophosphate
-
694847
Homo sapiens
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
-
694847
Methanococcus maripaludis
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
-
694847
Methanocaldococcus jannaschii
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
L-phosphoseryl-tRNA is the crucial precursor for L-selenocysteinyl-tRNA formation in archaea and eukarya. Selenocysteine formation is achieved by a two-step process: O-phosphoseryl-tRNASec kinase (PSTK) phosphorylates the endogenous Ser-tRNASec to O-phosphoseryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase (SepSecS)
694847
Methanococcus maripaludis
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
L-phosphoseryl-tRNA is the crucial precursor for L-selenocysteinyl-tRNA formation in archaea and eukarya. Selenocysteine formation is achieved by a two-step process: O-phosphoseryl-tRNASec kinase (PSTK) phosphorylates the endogenous Ser-tRNASec to O-phosphoseryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase (SepSecS)
694847
Methanocaldococcus jannaschii
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
O-phospho-L-seryl-tRNASec + selenophosphate
L-phosphoseryl-tRNA is the crucial precursor for L-selenocysteinyl-tRNA formation in archaea and eukarya. Selenocysteine formation is achieved by a two-step process: O-phosphoseryl-tRNASec kinase phosphorylates the endogenous L-seryl-tRNASec to O-phospho-L-seryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase
694847
Homo sapiens
L-selenocysteinyl-tRNASec + phosphate
-
-
-
?
Other publictions for EC 2.9.1.2
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738683
Manhas
Leishmania donovani encodes a ...
Leishmania donovani, Leishmania donovani Bob
J. Biol. Chem.
291
1203-1220
2016
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739263
Zhao
The role of Sep (O-phosphoseri ...
Homo sapiens
Placenta
34
967-972
2013
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723559
Li
Priority in selenium homeostas ...
Gallus gallus
PLoS ONE
7
e35761
2012
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1
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1
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723214
Hohn
Genetic analysis of selenocyst ...
Methanococcus maripaludis
Mol. Microbiol.
81
249-258
2011
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4
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1
1
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694074
Aeby
The selenoproteome is dispensa ...
Trypanosoma brucei
Mol. Biochem. Parasitol.
168
191-193
2009
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694917
Aeby
The canonical pathway for sele ...
Trypanosoma brucei
Proc. Natl. Acad. Sci. USA
106
5088-5092
2009
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6
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695136
Palioura
The human SepSecS-tRNASec comp ...
Homo sapiens
Science
325
321-325
2009
-
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1
7
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1
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2
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7
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694428
Araiso
Structural insights into RNA-d ...
Methanococcus maripaludis
Nucleic Acids Res.
36
1187-1199
2008
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1
1
5
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5
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1
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1
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694847
Yuan
RNA-dependent conversion of ph ...
Homo sapiens, Methanocaldococcus jannaschii, Methanococcus maripaludis
Proc. Natl. Acad. Sci. USA
103
18923-18927
2006
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