Application | Comment | Organism |
---|---|---|
analysis | methanogen diversity evidenced by molecular characterization of methyl coenzyme M reductase A genes in hydrothermal sediments of the Guaymas Basin | uncultured soil bacterium |
Organism | UniProt | Comment | Textmining |
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uncultured soil bacterium | - |
the methanogenic community in hydrothermally active sediments of Guaymas Basin (Gulf of California, Mexico) is analyzed by PCR amplification, cloning, and sequencing of methyl coenzyme M reductase genes. Members of the Methanomicrobiales and Methanosarcinales dominate the mcrA clone libraries from the upper 15 cm of the sediments. Within the H2 /CO2 - and formate-utilizing family Methanomicrobiales, two mcrA and 16S rRNA lineages are closely affiliated with cultured species of the genera Methanoculleus and Methanocorpusculum. The most frequently recovered mcrA PCR amplicons within the Methanomicrobiales does not branch with any cultured genera. Within the nutritionally versatile family Methanosarcinales, one 16S rRNA amplicon and most of the mcrA PCR amplicons are affiliated with the obligately acetate utilizing species Methanosaeta concilii. The mcrA clone libraries also includes phylotypes related to the methyl-disproportionating genus Methanococcoides. Two mcrA and two 16S rRNA lineages within the Methanosarcinales are unrelated to any cultured genus | - |
Synonyms | Comment | Organism |
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methyl coenzyme M reductase A | - |
uncultured soil bacterium |