Literature summary for 2.8.3.3 extracted from

  • Takamura, Y.; Kitayama, Y.
    Purification and some properties of malonate decarboxylase from Pseudomonas ovalis: an oligomeric enzyme with bifunctional properties (1981), Biochem. Int., 3, 483-491.
No PubMed abstract available

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
170000
-
gel filtration Pseudomonas putida
70000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE Pseudomonas putida
40000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE Pseudomonas putida
30000
-
1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + malonate Pseudomonas putida inducible enzyme acetate + malonyl-CoA
-
?
acetyl-CoA + malonate Pseudomonas putida IAM 1177 inducible enzyme acetate + malonyl-CoA
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
IAM 1177
-
Pseudomonas putida IAM 1177
-
IAM 1177
-

Purification (Commentary)

Purification (Comment) Organism
partial Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information succinyl-CoA or palmityl-CoA cannot replace acetyl-CoA Pseudomonas putida ?
-
?
additional information succinyl-CoA or palmityl-CoA cannot replace acetyl-CoA Pseudomonas putida IAM 1177 ?
-
?
acetyl-CoA + malonate absolutely dependent on acetyl-CoA Pseudomonas putida acetate + malonyl-CoA
-
?
acetyl-CoA + malonate inducible enzyme Pseudomonas putida acetate + malonyl-CoA
-
?
acetyl-CoA + malonate absolutely dependent on acetyl-CoA Pseudomonas putida IAM 1177 acetate + malonyl-CoA
-
?
acetyl-CoA + malonate inducible enzyme Pseudomonas putida IAM 1177 acetate + malonyl-CoA
-
?
malonyl-CoA + malonate exchange reaction Pseudomonas putida malonyl-CoA + malonate
-
?
methylmalonyl-CoA + malonate
-
Pseudomonas putida malonyl-CoA + methylmalonate
-
?
propionyl-CoA + malonate
-
Pseudomonas putida malonyl-CoA + propanoate
-
?
butyryl-CoA + malonate
-
Pseudomonas putida malonyl-CoA + butanoate
-
?

Subunits

Subunits Comment Organism
tetramer 1 * 70000 + 1 * 40000 + 2 * 30000, alphabeta1(beta2)2, SDS-PAGE Pseudomonas putida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Pseudomonas putida