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Literature summary for 2.8.3.21 extracted from
- Redirecting metabolic fluxes through cofactor engineering: Role of CoA-esters pool during L(-)-carnitine production by Escherichia coli (2007), J. Biotechnol., 132, 110-117.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | production of L-carnithine in Escherichia coli. The overexpression of CoA-transferase CaiB and CoA-ligase CaiC leads to an increase in L-carnitine production. Under optimal concentrations of inducer and fumarate yields reach 10- and 50fold, respectively, that obtained for the wild type strain. Low levels of coenzyme A limit the activity of these two enzymes. The glyoxylate shunt and anaplerotic pathways limit the bioprocess since strains carrying deletions of isocitrate lyase and isocitrate dehydrogenase phosphatase/kinase yielded 20-25% more L-carnitine than the control. The deletion of phosphotransacetylase strongly inhibits the bioprocess | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
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Release 2023.1 (January 2023)
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