Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | increases activity | Rattus norvegicus |
General Stability | Organism |
---|---|
addition of 3mg/ml bovine serum albumin slightly increases stability, 10% glycerol stabilizes | Rattus norvegicus |
mercaptoethanol improves stability | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetate | non-competitive inhibition | Rattus norvegicus | |
malonate | competitive inhibition | Rattus norvegicus | |
succinate | competitive inhibition of the product | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
succinyl-CoA | pH 8.5, 30°C, cosubstrate 3-hydroxy-3-methylglutarate | Rattus norvegicus | |
0.37 | - |
malonyl-CoA | pH 8.5, 30°C, cosubstrate 3-hydroxy-3-methylglutarate | Rattus norvegicus | |
1.7 | - |
3-hydroxy-3-methylglutarate | pH 8.5, 30°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | mainly in the mitochondrial matrix, also in the inner membrane | Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + 3-hydroxy-3-methylglutarate | Rattus norvegicus | - |
succinate + 3-hydroxy-3-methylglutaryl-CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar albino | - |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0194 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, enzyme preparation with glycerol and mercaptoethanol retains 80% of its activity after 2 week of storage | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyryl-CoA + 3-hydroxy-3-methylglutarate | only negligible amounts of HMGCoA produced | Rattus norvegicus | butyrate + 3-hydroxy-3-methylglutaryl-CoA | - |
? | |
malonyl-CoA + 3-hydroxy-3-methylglutarate | - |
Rattus norvegicus | malonate + 3-hydroxy-3-methylglutaryl-CoA | - |
r | |
succinyl-CoA + 3-hydroxy-3-methylglutarate | - |
Rattus norvegicus | succinate + 3-hydroxy-3-methylglutaryl-CoA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
succinylCoA:3-hydroxy-3-methylglutarate coenzyme A transferase | - |
Rattus norvegicus |
succinylCoA:HMG coenzyme A transferase | - |
Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
decreasing the pH to lower values causes an irreversible loss of the enzyme activity | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
acetoacetate | pH 8.5, 30°C | Rattus norvegicus | |
1.5 | - |
succinate | pH 8.5, 30°C | Rattus norvegicus | |
2.1 | - |
malonate | pH 8.5, 30°C | Rattus norvegicus |