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Literature summary for 2.8.2.7 extracted from

  • Harada, T.; Shimizu, S.; Nakanishi, Y.; Suzuki, S.
    Enzymatic transfer of sulfate from 3-phosphoadenosine 5-phosphosulfate to uridine diphosphate N-acetylgalactosamine 4-sulfate (1967), J. Biol. Chem., 242, 2288-2294.
    View publication on PubMed
Search for more literature for 2.8.2.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 UDP-N-acetylgalactosamine 4-sulfate pH 4.8, 38°C Gallus gallus
0.13
-
 N-acetylgalactosamine 1-phosphate 4-sulfate pH 4.8, 38°C Gallus gallus
1.4
-
 N-acetylgalactosamine 4-sulfate pH 4.8, 38°C Gallus gallus
2
-
 DELTA4,5-glucuronido-N-acetylgalactosamine 4-sulfate pH 4.8, 38°C Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble
-
 Gallus gallus
-
-

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
 White Leghorn hen
-

Purification (Commentary)

Purification (Comment) Organism
partial Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
oviduct predominantly in tubular glands and isthmus Gallus gallus
-
oviduct distribution in magnum Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0000069
-
-
 Gallus gallus

Storage Stability

Storage Stability Organism
-18°C, 1 month Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylylsulfate + chondroitin poor substrate Gallus gallus ?
-
 ?
3'-phosphoadenylylsulfate + delta4,5-glucuronido-N-acetylgalactosamine 4-sulfate poor substrate Gallus gallus ?
-
 ?
3'-phosphoadenylylsulfate + N-acetylgalactosamine 1-phosphate 4-sulfate
-
 Gallus gallus adenosine 3',5'-bisphosphate + N-acetylgalactosamine 1,6-bissulfate
-
 ?
3'-phosphoadenylylsulfate + N-acetylgalactosamine 4-sulfate
-
 Gallus gallus adenosine 3',5'-bisphosphate + N-acetylgalactosamine 4,6-bissulfate
-
 ?
3'-phosphoadenylylsulfate + UDP-N-acetylgalactosamine 4-sulfate i.e. 3'-phosphoadenosine 5'-phosphosulfate or PAPS, catalyses the transfer of sulfate to position 6 of N-acetylgalactosamine moiety of UDP-N-acetylgalactosamine sulfate Gallus gallus adenosine 3',5'-bisphosphate + UDP-N-acetylgalactosamine-4,6-bissulfate
-
 ?
additional information no substrates are N-acetylgalactosamine, UDP-N-galactosamine, chondrosin, delta4,5-glucuronido-N-acetylgalactosamine, N-acetylgalactosamine 6-sulfate, delta4,5-glucuronido-N-acetylgalactosamine 6-sulfate, chondroitin sulfate A, B or C, oversulfated chondroitin sulfate from shark cartilage, hyaluronic acid, kerato sulfate or heparin Gallus gallus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
38
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
-
 Gallus gallus