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Literature summary for 2.8.2.30 extracted from

  • Copeland, R.; Balasubramaniam, A.; Tiwari, V.; Zhang, F.; Bridges, A.; Linhardt, R.J.; Shukla, D.; Liu, J.
    Using a 3-O-sulfated heparin octasaccharide to inhibit the entry of herpes simplex virus type 1 (2008), Biochemistry, 47, 5774-5783.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.2.30

Application

Application Comment Organism
drug development introduction of a single sulfate group into the heparin octasaccharide is sufficient for effective blocking of HSV-1 infection Human alphaherpesvirus 1

Cloned(Commentary)

Cloned (Comment) Organism
expression of N-terminally His6-tagged 3-OST-3 catalytic domain, comprising residues Gly139-Gly406, in Escherichia coli Human alphaherpesvirus 1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Human alphaherpesvirus 1 heparan sulfate is a highly sulfated polysaccharide and is present in large quantities on the cell surface and in the extracellular matrix. Herpes simplex virus type 1 utilizes a specialized cell surface HS, known as 3-O-sulfated HS, as an entry receptor to establish infection. 3-O-sulfated octasaccharide has strong activity in blocking HSV-1 infection, stronger than that of the 3-OH octasaccharide, overview ?
-
 ?
additional information Human alphaherpesvirus 1 HSV-1 heparan sulfate is a highly sulfated polysaccharide and is present in large quantities on the cell surface and in the extracellular matrix. Herpes simplex virus type 1 utilizes a specialized cell surface HS, known as 3-O-sulfated HS, as an entry receptor to establish infection. 3-O-sulfated octasaccharide has strong activity in blocking HSV-1 infection, stronger than that of the 3-OH octasaccharide, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Human alphaherpesvirus 1
-
 HSV-1
-
Human alphaherpesvirus 1 HSV-1
-
 HSV-1
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged 3-OST-3 catalytic domain, comprising residues Gly139-Gly406, from Escherichia coli Human alphaherpesvirus 1

Source Tissue

Source Tissue Comment Organism Textmining
additional information infection of human HeLa and cornea fibroblast cells Human alphaherpesvirus 1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
heparin 3-hydroxy octasaccharide + 3'-phosphoadenylyl sulfate i.e. 3-OH octasaccharide, from partially depolymerized heparin, substrate preparation: upscaling by by coupling 3-OST-3 modification with a PAPS regeneration system, overview. Determination of the site of 3-O-sulfation in the octasaccharide at the G6 residue Human alphaherpesvirus 1 3-O-sulfated heparin octasaccharide + adenosine 3',5'-bisphosphate i.e. DELTAUA2S-GlcNS6S-IdoUA2S-GlcNS6S-IdoUA2S-GlcNS3S6S-IdoUA2SGlcNS6S, where DELTAUA is 4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid, GlcN is D-glucosamine, and IdoUA is L-iduronic acid ?
heparin 3-hydroxy octasaccharide + 3'-phosphoadenylyl sulfate i.e. 3-OH octasaccharide, from partially depolymerized heparin, substrate preparation: upscaling by by coupling 3-OST-3 modification with a PAPS regeneration system, overview. Determination of the site of 3-O-sulfation in the octasaccharide at the G6 residue Human alphaherpesvirus 1 HSV-1 3-O-sulfated heparin octasaccharide + adenosine 3',5'-bisphosphate i.e. DELTAUA2S-GlcNS6S-IdoUA2S-GlcNS6S-IdoUA2S-GlcNS3S6S-IdoUA2SGlcNS6S, where DELTAUA is 4-deoxy-alpha-L-threo-hex-4-enopyranosyluronic acid, GlcN is D-glucosamine, and IdoUA is L-iduronic acid ?
additional information heparan sulfate is a highly sulfated polysaccharide and is present in large quantities on the cell surface and in the extracellular matrix. Herpes simplex virus type 1 utilizes a specialized cell surface HS, known as 3-O-sulfated HS, as an entry receptor to establish infection. 3-O-sulfated octasaccharide has strong activity in blocking HSV-1 infection, stronger than that of the 3-OH octasaccharide, overview Human alphaherpesvirus 1 ?
-
 ?
additional information heparan sulfate is a highly sulfated polysaccharide and is present in large quantities on the cell surface and in the extracellular matrix. Herpes simplex virus type 1 utilizes a specialized cell surface HS, known as 3-O-sulfated HS, as an entry receptor to establish infection. 3-O-sulfated octasaccharide has strong activity in blocking HSV-1 infection, stronger than that of the 3-OH octasaccharide, overview Human alphaherpesvirus 1 HSV-1 ?
-
 ?

Synonyms

Synonyms Comment Organism
3-OST-3
-
 Human alphaherpesvirus 1
HS 3-O-sulfotransferase isoform 3
-
 Human alphaherpesvirus 1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Human alphaherpesvirus 1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Human alphaherpesvirus 1