BRENDA - Enzyme Database show
show all sequences of 2.8.2.30

The heparin/heparan sulfate sequence that interacts with cyclophilin B contains a 3-O-sulfated N-unsubstituted glucosamine residue

Vanpouille, C.; Deligny, A.; Delehedde, M.; Denys, A.; Melchior, A.; Lienard, X.; Lyon, M.; Mazurier, J.; Fernig, D.G.; Allain, F.; J. Biol. Chem. 282, 24416-24429 (2007)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
additional information
a synthetic siRNA duplex, si3-OST-3, corresponding to 3-OST-3 mRNA sequences is used to inhibit the expression of mRNAs encoding 3-OST-3 isoforms, downregulation of the expression of 3-OST-3 by RNA interference potently reduced CyPB binding and consequent activation of p44/42 mitogen-activated protein kinases, overview
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
cyclosporine B heparin binding kinetics, overview
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
Golgi apparatus
-
Homo sapiens
5794
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
Homo sapiens
3-O-sulfation of GlcNH2-containing heparan sulfate by 3-OST-3 provides binding sites for glycoprotein gD of Herpes simplex virus type I and is involved in involved in cyclosporine B, CyPB, binding and viral infection, analysis of heparin sulfate structures involve, overview
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
JURKAT cell
clone E6-1,expresssion of 3-OST-3B isoform
Homo sapiens
-
T-lymphocyte
of peripheral blood, expresssion of 3-OST-3B isoform
Homo sapiens
-
THP-1 cell
promonocytic leukemia cells
Homo sapiens
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
p44/42 MAPK activation, semi-quantitative expression analysis by RT-PCR, determination of unmodified heparin and cell surface binding, overview
Homo sapiens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
3-O-sulfation of GlcNH2-containing heparan sulfate by 3-OST-3 provides binding sites for glycoprotein gD of Herpes simplex virus type I and is involved in involved in cyclosporine B, CyPB, binding and viral infection, analysis of heparin sulfate structures involve, overview
674849
Homo sapiens
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
C5 epimerization of some GlcUA into L-iduronic acid, IdoUA, 2-O-sulfation of IdoUA, and 6-O-sulfation of GlcN units
674849
Homo sapiens
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
-
-
?
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
a synthetic siRNA duplex, si3-OST-3, corresponding to 3-OST-3 mRNA sequences is used to inhibit the expression of mRNAs encoding 3-OST-3 isoforms, downregulation of the expression of 3-OST-3 by RNA interference potently reduced CyPB binding and consequent activation of p44/42 mitogen-activated protein kinases, overview
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
cyclosporine B heparin binding kinetics, overview
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
Golgi apparatus
-
Homo sapiens
5794
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
Homo sapiens
3-O-sulfation of GlcNH2-containing heparan sulfate by 3-OST-3 provides binding sites for glycoprotein gD of Herpes simplex virus type I and is involved in involved in cyclosporine B, CyPB, binding and viral infection, analysis of heparin sulfate structures involve, overview
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
JURKAT cell
clone E6-1,expresssion of 3-OST-3B isoform
Homo sapiens
-
T-lymphocyte
of peripheral blood, expresssion of 3-OST-3B isoform
Homo sapiens
-
THP-1 cell
promonocytic leukemia cells
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
additional information
-
p44/42 MAPK activation, semi-quantitative expression analysis by RT-PCR, determination of unmodified heparin and cell surface binding, overview
Homo sapiens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
3-O-sulfation of GlcNH2-containing heparan sulfate by 3-OST-3 provides binding sites for glycoprotein gD of Herpes simplex virus type I and is involved in involved in cyclosporine B, CyPB, binding and viral infection, analysis of heparin sulfate structures involve, overview
674849
Homo sapiens
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
-
-
?
3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine
C5 epimerization of some GlcUA into L-iduronic acid, IdoUA, 2-O-sulfation of IdoUA, and 6-O-sulfation of GlcN units
674849
Homo sapiens
adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate
-
-
-
?
Other publictions for EC 2.8.2.30
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738746
Sikora
Regulation of the expression o ...
Homo sapiens
J. Cell. Biochem.
117
1529-1542
2016
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1
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1
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1
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739207
Mao
The heparan sulfate sulfotrans ...
Homo sapiens
Oncogene
35
5043-5055
2016
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1
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9
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1
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1
1
1
1
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737733
Zhao
Heparan sulfate 6-O-sulfotrans ...
Rattus norvegicus
Biochemistry
80
379-389
2015
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1
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738745
Zhang
Heparan sulfate D-glucosaminyl ...
Homo sapiens
J. Cell. Biochem.
116
1101-1112
2015
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1
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739264
Amraoui
Placental expression of hepara ...
Homo sapiens
Placenta
36
1218-1224
2015
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738938
Yakoub
Comprehensive analysis of herp ...
Danio rerio
J. Virol.
88
12915-12922
2014
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738961
Thacker
Heparan sulfate 3-O-sulfation: ...
Homo sapiens
Matrix Biol.
35
60-72
2014
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10
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10
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726083
Choudhary
Role of filopodia in HSV-1 Ent ...
Danio rerio
Open Virol. J.
7
41-48
2013
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1
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724808
Liu
Understanding the substrate sp ...
Homo sapiens
Curr. Opin. Struct. Biol.
22
550-557
2012
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725947
Nguyen
A synthetic heparan sulfate ol ...
Homo sapiens
Mol. Biosyst.
8
609-614
2012
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1
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726389
Moon
Dissecting the substrate recog ...
Mus musculus
Proc. Natl. Acad. Sci. USA
109
5265-5270
2012
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5
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724692
Song
Heparan sulfate D-glucosaminyl ...
Homo sapiens
Cancer Biol. Ther.
12
388-398
2011
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703620
Bui
Epigenetics: methylation-assoc ...
Homo sapiens
FASEB J.
24
436-450
2010
1
1
1
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726527
Zhang
Heparin sulphate D-glucosaminy ...
Homo sapiens
Virology
406
280-285
2010
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726544
Hubbard
Zebrafish-encoded 3-O-sulfotra ...
Danio rerio
Zebrafish
7
181-187
2010
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1
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690965
Copeland
Using a 3-O-sulfated heparin o ...
Human alphaherpesvirus 1, Human alphaherpesvirus 1 HSV-1
Biochemistry
47
5774-5783
2008
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1
1
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674849
Vanpouille
The heparin/heparan sulfate se ...
Homo sapiens
J. Biol. Chem.
282
24416-24429
2007
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1
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691054
Wille
Biophysical investigation of h ...
Homo sapiens
Biochim. Biophys. Acta
1774
1470-1476
2007
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1
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1
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1
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2
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1
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2
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673246
Cadwallader
Combinatorial expression patte ...
Danio rerio
Dev. Dyn.
235
3423-3431
2006
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1
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675705
Tiwari
Role for 3-O-sulfated heparan ...
Homo sapiens
J. Virol.
80
8970-8980
2006
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2
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1
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677244
O'Donnell
A role for heparan sulfate 3-O ...
Homo sapiens
Virology
346
452-459
2006
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394962
Shworak
Multiple isoforms of heparan s ...
Homo sapiens
J. Biol. Chem.
274
5170-5184
1999
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4
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394963
Liu
Expression of heparan sulfate ...
Homo sapiens, Mus musculus
J. Biol. Chem.
274
5185-5192
1999
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1
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394964
Liu
Heparan sulfate D-glucosaminyl ...
Homo sapiens
J. Biol. Chem.
274
38155-38162
1999
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1
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1
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394965
Shukla
A novel role for the 3-O-sulfa ...
Homo sapiens, Mus musculus
Cell
99
13-22
1999
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2
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