Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | trans-zeatin induces AtST3a enzyme expression | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
AtST3a, DNA and amino acid sequence determination and analysis, expression of the His-tagged enzyme in Escherichia coli strain XL-1 Blue | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
adenosine 3'-phosphate 5'-phosphosulfate | pH 7.5, 25°C, recombinant enzyme | Arabidopsis thaliana | |
0.002 | - |
kaempferol 3-sulfate | pH 7.5, 25°C, recombinant enzyme | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine 3'-phosphate 5'-phosphosulfate + kaempferol 3-sulfate | Arabidopsis thaliana | - |
adenosine 3',5'-bisphosphate + kaempferol 3,7-bissulfate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Col0 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged AtST3a from Escherichia coli strain XL-1 Blue by nickel affinity and on 3'-phosphoadenosine 5'-phosphate affinity chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
inflorescence stalk | - |
Arabidopsis thaliana | - |
additional information | no expression in leaves of of 15-day-old plants, developmental expression pattern, the AtST3a transcript cannot be detected in extracts of wounded or etiolated plant material | Arabidopsis thaliana | - |
root | root-specific expression of AtST3a in roots of 15-day-old plants | Arabidopsis thaliana | - |
seedling | ST3a is expressed at early stages of seedling development | Arabidopsis thaliana | - |
silique | - |
Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.017 | - |
purified recombinant enzyme, substrate kaempferol 3-sulfate | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
adenosine 3'-phosphate 5'-phosphosulfate + 3-hydroxyflavone | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 4-oxo-2-phenyl-4H-chromen-3-yl hydrogen sulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + 7-hydroxyflavone | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + 7-hydroxyflavone 7-sulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + apigenin | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + apigenin 7-sulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + apigenin 4'-sulfate | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + apigenin 4',7-bissulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + crysin | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + crysin 7-sulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + dihydroquercetin | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + dihydroquercetin 7-sulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + galangin | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + galangin 7-sulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + genistein | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + genistein 7-sulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + gossypetin | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + gossypetin 7-sulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + isorhamnetin | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + isorhamnetin 7-sulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + kaempferol | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + kaempferol 7-sulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + kaempferol 3-sulfate | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + kaempferol 3,7-bissulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + kaempferol 3-sulfate | best substrate | Arabidopsis thaliana | adenosine 3',5'-bisphosphate + kaempferol 3,7-bissulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + myricetin | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + myricetin 7-sulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + quercetin | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + quercetin 7-sulfate | - |
? | |
adenosine 3'-phosphate 5'-phosphosulfate + rhamnetin | - |
Arabidopsis thaliana | adenosine 3',5'-bisphosphate + ? | - |
? | |
additional information | substrate specificity, overview, no activity with quercetagetin, AtST3a isozyme exhibits strict specificity for position 7 of flavonoids, and accept as substrates a number of flavonols and flavone aglycones, as well as their monosulfate esters | Arabidopsis thaliana | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AtST3a | - |
Arabidopsis thaliana |
flavonoid 7-sulfotransferase | - |
Arabidopsis thaliana |
flavonol 7-ST | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Arabidopsis thaliana |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Arabidopsis thaliana |