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Literature summary for 2.8.2.24 extracted from

  • Jain, J.C.; Groot Wassink, J.W.D.; Kolenovsky, A.D.; Underhill, E.W.
    Purification and properties of 3'-phosphoadenosine 5'-phosphosulfate: desulphoglucosinolate sulphotransefrase from Brassica juncea cell cultures (1990), Phytochemistry, 29, 1425-1428.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
adenosine-3',5'-bisphosphate competitive to 3'-phosphoadenylylsulfate, kinetics Brassica juncea
Co2+ at low concentration Brassica juncea
Cu2+ at low concentration Brassica juncea
iodoacetamide
-
Brassica juncea
iodoacetic acid weak inhibition Brassica juncea
Iodosobenzoic acid
-
Brassica juncea
Mn2+ inhibitory at 10 mM, stimulating at 1 mM Brassica juncea
additional information no inhibition by 3'-AMP, 5'-AMP, 2',5'-AMP, 3',5'-cAMP, 5'-ADP, 5'-ATP, adenosine-5'-phosphosulfate, benzaldoxime, phenylacetohydroximate, phenylacetothiohydroximate, S-methylphenylacetothiohydroximate, benzylglucosinolate Brassica juncea
N-Methylmaleimide
-
Brassica juncea
N-Pyrenylmaleimide
-
Brassica juncea
PCMB
-
Brassica juncea
Phenylmercuriacetate
-
Brassica juncea
Zn2+ at low concentration Brassica juncea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00078
-
3'-phosphoadenylylsulfate pH 8.5-9.0, 30┬░C Brassica juncea
0.0023
-
desulfobenzylglucosinolate pH 8.5-9.0, 30┬░C Brassica juncea

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ slight activation Brassica juncea
Mn2+ 23% activation at 1 mM, 10 mM inhibits by 53% Brassica juncea
additional information no absolute requirement for cations Brassica juncea

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
gel filtration Brassica juncea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate Brassica juncea
-
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
additional information Brassica juncea involved in the biosynthesis of glucosinolates ?
-
?
additional information Brassica juncea involved with EC 2.4.1.195 in final steps of thioglycoside biosynthesis in cruciferous plants ?
-
?

Organism

Organism UniProt Comment Textmining
Brassica juncea
-
cv. Cutlass
-

Purification (Commentary)

Purification (Comment) Organism
partial, 230fold Brassica juncea

Source Tissue

Source Tissue Comment Organism Textmining
cell culture derived from hypocotyls Brassica juncea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
0.0000329
-
partially purified enzyme Brassica juncea

Storage Stability

Storage Stability Organism
-20┬░C, 20 mM Tris buffer, pH 7.5, 14 mM 2-mercaptoethanol, 10% glycerol, at least 2 months Brassica juncea
4┬░C, 14 mM 2-mercaptoethanol, t1/2: about 2 weeks Brassica juncea
4┬░C, more than 50% loss of activity within 48 h Brassica juncea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Brassica juncea adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
-
Brassica juncea adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
additional information no activity with adenosine-5'-phosphosulfate as donor Brassica juncea ?
-
?
additional information involved in the biosynthesis of glucosinolates Brassica juncea ?
-
?
additional information involved with EC 2.4.1.195 in final steps of thioglycoside biosynthesis in cruciferous plants Brassica juncea ?
-
?

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
-
Brassica juncea

Temperature Range [┬░C]

Temperature Minimum [┬░C] Temperature Maximum [┬░C] Comment Organism
25 40 about 85% of maximal activity at 25┬░C and about half-maximal activity at 40┬░C Brassica juncea

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
40
-
at least 1 h, inactivation above Brassica juncea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5 9 maximal activity in Tris-HCl buffer Brassica juncea

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0004
-
adenosine-3',5'-bisphosphate pH 8.5-9.0, 30┬░C Brassica juncea

pI Value

Organism Comment pI Value Maximum pI Value
Brassica juncea
-
-
4.84