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Literature summary for 2.8.2.24 extracted from

  • Jain, J.C.; Groot Wassink, J.W.D.; Reed, D.W.; Underhill, E.W.
    Persistent co-purification of enzymes catalyzing the sequential glucosylation and sulfation steps in glucosinolate biosynthesis (1990), J. Plant Physiol., 136, 356-361.
No PubMed abstract available

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Brassica juncea 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate Brassica juncea
-
adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
additional information Brassica juncea involved with EC 2.4.1.195 in final steps of thioglycoside biosynthesis in cruciferous plants ?
-
?

Organism

Organism UniProt Comment Textmining
Brassica juncea
-
cv. Cutlass
-

Purification (Commentary)

Purification (Comment) Organism
persistently co-purified with EC 2.4.1.195 Brassica juncea

Source Tissue

Source Tissue Comment Organism Textmining
cell culture derived from hypocotyls Brassica juncea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-phosphoadenylyl sulfate + desulfobenzylglucosinolate absolute specificity for desulfoglucosinolate structure Brassica juncea adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
3'-phosphoadenylylsulfate + desulfobenzylglucosinolate
-
Brassica juncea adenosine 3',5'-bisphosphate + benzylglucosinolate
-
?
additional information involved with EC 2.4.1.195 in final steps of thioglycoside biosynthesis in cruciferous plants Brassica juncea ?
-
?

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
40
-
up to, stable Brassica juncea
45
-
t1/2: 1 h, 20 mM Tris-HCl buffer, pH 7.5, 14 mM 2-mercaptoethanol Brassica juncea

pH Stability

pH Stability pH Stability Maximum Comment Organism
6
-
t1/2: 1 h, 4┬░C Brassica juncea
8
-
stable above Brassica juncea
10.5
-
t1/2: 1 h, 4┬░C Brassica juncea