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Literature summary for 2.8.2.22 extracted from
- Cloning, expression and purification of arylsulfate sulfotransferase from Eubacterium A-44 (2007), Biol. Pharm. Bull., 30, 11-14.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene astA, cloned from a genomic library, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain TH2, expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) | Eubacterium sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
x * 75000, cleaved recombinant enzyme, SDS-PAGE | Eubacterium sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Eubacterium sp. | - |
- |
- |
| Eubacterium sp. A-44 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) by treatement with enterokinase to exclude the DsbA and nickel affinity chromatography | Eubacterium sp. |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.6 | - |
purified, recombinant, enterokinase-cleaved enzyme | Eubacterium sp. |
| 37.1 | - |
purified recombinant enzyme | Eubacterium sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl sulfate + 4-hydroxybenzoate | low activity | Eubacterium sp. | 4-nitrophenol + 4-hydroxybenzoyl sulfate | - |
? |  |
| 4-nitrophenyl sulfate + 4-hydroxybenzoate | low activity | Eubacterium sp. A-44 | 4-nitrophenol + 4-hydroxybenzoyl sulfate | - |
? | |
| 4-nitrophenyl sulfate + tyramine | best acceptor substrate | Eubacterium sp. | 4-nitrophenol + tyramine sulfate | - |
? | |
| 4-nitrophenyl sulfate + tyramine | best acceptor substrate | Eubacterium sp. A-44 | 4-nitrophenol + tyramine sulfate | - |
? | |
| 4-nitrophenyl sulfate + tyrosine | low activity | Eubacterium sp. | 4-nitrophenol + tyrosine sulfate | - |
? | |
| 4-nitrophenyl sulfate + tyrosine | low activity | Eubacterium sp. A-44 | 4-nitrophenol + tyrosine sulfate | - |
? | |
| additional information | the enzyme transfers a sulfate group from phenolic sulfate esters to phenolic acceptors | Eubacterium sp. | ? | - |
? | |
| additional information | the enzyme transfers a sulfate group from phenolic sulfate esters to phenolic acceptors | Eubacterium sp. A-44 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000, cleaved recombinant enzyme, SDS-PAGE | Eubacterium sp. |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| arylsulfate sulfotransferase | - |
Eubacterium sp. |
| ASST | - |
Eubacterium sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Eubacterium sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Eubacterium sp. |
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