Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Deoxy-3'-phosphoadenosine 5'-phosphate | - |
Rattus norvegicus | |
3'-phosphoadenosine 5'-phosphate | - |
Rattus norvegicus | |
guanosine 3',5'-diphosphate | - |
Rattus norvegicus | |
Mg2+ | 1-10 mM: enhances activity, 20-35 mM: inhibition | Rattus norvegicus | |
Mn2+ | 1-10 mM: enhances activity, 20-35 mM: inhibition | Rattus norvegicus | |
additional information | not: 2'-phosphoadenosine 5'-phosphate, fluorosulfonylbenzoyladenosine | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0052 | - |
3'-phosphoadenylylsulfate | pH 6.2, 37°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1-10 mM and 20-35 mM: enhancement | Rattus norvegicus | |
Mg2+ | 1-10 mM: enhances activity, 20-35 mM: inhibition | Rattus norvegicus | |
Mn2+ | 1-10 mM: enhances activity, 20-35 mM: inhibition | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
nondenaturing PAGE | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | enzyme regulates the sulfation of glycolipids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | purified enzyme contains bound lipids, consisting primarily of cholesterol and phosphatidylcholine, removal of associated lipids results in loss of activity | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 0.1% Triton X-100, pH 7.0-7.5, stable for more than 4 weeks | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
galactosylceramide + 3'-phosphoadenylylsulfate | biotinylated and light-sensitive azido derivatives of lysogalactosylceramide are synthesized by the crude enzyme preparation, these derivatives remain effective substrates for the testicular enzyme | Rattus norvegicus | galactosylceramide sulfate + adenosine 3',5'-bisphosphate | - |
? | |
additional information | enzyme regulates the sulfation of glycolipids | Rattus norvegicus | ? | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, complete loss of activity | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6 | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
guanosine 3',5'-diphosphate | pH 6.2, 37°C | Rattus norvegicus | |
0.3 | - |
3'-phosphoadenosine 5'-phosphate | pH 6.2, 37°C | Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | isoelectric foccusing | - |
5.1 |