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Literature summary for 2.8.1.7 extracted from

  • Marelja, Z.; Mullick Chowdhury, M.; Dosche, C.; Hille, C.; Baumann, O.; Loehmannsroeben, H.G.; Leimkuehler, S.
    The L-cysteine desulfurase NFS1 is localized in the cytosol where it provides the sulfur for molybdenum cofactor biosynthesis in humans (2013), PLoS ONE, 8, e60869.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Homo sapiens 5829
-
mitochondrion predominantly localized in the mitochondria Homo sapiens 5739
-
nucleus
-
 Homo sapiens 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 2 * 45000, SDS-PAGE Homo sapiens
50000
-
 x * 50000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-cysteine + MOC3 protein Homo sapiens
-
 L-alanine + S-sulfanyl-MOC3 protein
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HeLa cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-cysteine + MOC3 protein
-
 Homo sapiens L-alanine + S-sulfanyl-MOC3 protein
-
 ?
L-cysteine + [enzyme]-cysteine
-
 Homo sapiens L-alanine + [enzyme]-S-sulfanylcysteine
-
 ?
additional information NFS1 acts as the sulfur donor for the C-terminal domain of MOCS3 protein (a cytosolic protein involved in molybdenum cofactor biosynthesis and tRNA thiolation) Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Homo sapiens
homodimer 2 * 45000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
L-cysteine desulfurase
-
 Homo sapiens
Nfs1
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Homo sapiens

General Information

General Information Comment Organism
metabolism the enzyme is the sulfur donor for M molybdenum cofactor biosynthesis. The enzyme is able to transfer sulfur to the C-terminal domain of MOCS3, a cytosolic protein involved in molybdenum cofactor biosynthesis and tRNA thiolation Homo sapiens
physiological function the enzyme provides the sulfur for molybdenum cofactor biosynthesis in human cytosol. The enzyme plays a crucial role in the mitochondrial iron-sulfur cluster biosynthesis and in the thiomodification of mitochondrial and cytosolic tRNAs Homo sapiens