Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cysteine | inhibits reaction with 3-mercaptopyruvate | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + 'activated' tRNA | Rattus norvegicus | - |
L-serine + tRNA containing a thionucleotide | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
Wistar albino | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Rattus norvegicus | - |
brain | cerebral cortex, lowest activity | Rattus norvegicus | - |
heart | - |
Rattus norvegicus | - |
kidney | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
lung | - |
Rattus norvegicus | - |
muscle | highest activity | Rattus norvegicus | - |
testis | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-60°C, instable on storage, overnight storage results in 50% lost of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-mercaptopyruvate + activated tRNA | reaction product is sensitive to deacylation | Rattus norvegicus | ? + S-tRNA | - |
? | |
L-cysteine + 'activated' tRNA | - |
Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
L-cysteine + Bos taurus 'activated' tRNA | calf liver tRNA better substrate than rat liver tRNA | Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
L-cysteine + E. coli 'activated' tRNA | sulfur deficient tRNA | Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
L-cysteine + Rattus norvegicus brain 'activated' tRNA | better substrate than liver tRNA | Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
L-cysteine + Rattus norvegicus liver 'activated' tRNA | poorest substrate | Rattus norvegicus | L-serine + tRNA containing a thionucleotide | - |
r | |
additional information | only tRNAs are effective sulfur acceptors, little or no reaction with rat liver rRNA, salmon sperm DNA, polyuridylic acid and polyadenylic acid | Rattus norvegicus | ? | - |
? |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 37 | at 10°C rate of sulfur transfer being 1/3 that at 37°C | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | about 75% of activity maximum at pH 7.0, about 55% of activity maximum at pH 9.0 | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | - |
inactivated | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |