Any feedback?
Literature summary for 2.7.9.4 extracted from
- Functional characterization of alpha-glucan,water dikinase, the starch phosphorylating enzyme (2004), Biochem. J., 377, 525-532.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Solanum tuberosum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H992A | mutant enzyme backgroun level of activity, no autophosphorylation | Solanum tuberosum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 330000 | - |
gel filtration | Solanum tuberosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | Q9AWA5 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | autophosphorylation of wild-type enzyme, no histidine autophosphorylation of mutant enzyme H992A | Solanum tuberosum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type and mutant enzyme H992A | Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + ATP + H2O | - |
Solanum tuberosum | AMP + phospho-amylopectin + phosphate | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | - |
Solanum tuberosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
- |
Solanum tuberosum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | 20°C: about 75% of maximal activity, 40°C: about 85% of maximal activity | Solanum tuberosum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
5 min, about 15% loss of activity | Solanum tuberosum |
| 50 | - |
5 min, about 40% loss of activity | Solanum tuberosum |
| 55 | - |
5 min, complete loss of activity | Solanum tuberosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Solanum tuberosum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
