Literature summary for 2.7.9.3 extracted from

Noinaj, N.; Wattanasak, R.; Lee, D.Y.; Wally, J.L.; Piszczek, G.; Chock, P.B.; Stadtman, T.C.; Buchanan, S.K.
Structural insights into the catalytic mechanism of Escherichia coli selenophosphate synthetase (2012), J. Bacteriol., 194, 499-508.

Search for more literature for 2.7.9.3

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
mutant enzyme C17S in apo form, hanging drop vapor diffusion method, using 50 mM Tris-HCl (pH 8.25), 50 mM MgCl2, and 32% (w/v) PEG MME 550	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
D227A	inactive	Escherichia coli
D51A	inactive	Escherichia coli
D68A	inactive	Escherichia coli
D91A	inactive	Escherichia coli
N87A	inactive	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	dependent on	Escherichia coli
Mg2+	dependent on	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	P16456	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-agarose column chromatography	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + selenide + H2O	-	Escherichia coli	AMP + selenophosphate + phosphate	-	?

Subunits

Subunits	Comment	Organism
dimer	sedimentation velocity analysis	Escherichia coli

Synonyms

Synonyms	Comment	Organism
selenophosphate synthetase	-	Escherichia coli
SPS	-	Escherichia coli

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Release 2023.1 (January 2023)