Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strains DH5alpha and BL21-CodonPlus(DE3)-RIL | Thermoproteus tenax |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | strong inhibition at 1 mM | Thermoproteus tenax | |
ADP | strong inhibition at 1 mM | Thermoproteus tenax | |
AMP | strong inhibition at 1 mM | Thermoproteus tenax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
pyruvate | in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C | Thermoproteus tenax | |
1 | - |
ATP | in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C | Thermoproteus tenax |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM, required | Thermoproteus tenax | |
additional information | activity of PEPS does not depend on monovalent cations such as K+ and NH4+ | Thermoproteus tenax |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
88000 | - |
SDS-PAGE, recombinant enzyme, phosphoenzyme intermediate form | Thermoproteus tenax |
90500 | - |
calculated from amino acid sequence | Thermoproteus tenax |
94000 | - |
SDS-PAGE, recombinant enzyme, nonphosphorylated form | Thermoproteus tenax |
600000 | - |
above 600000, gel filtration | Thermoproteus tenax |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoproteus tenax | Q70WQ8 | - |
- |
Thermoproteus tenax Kra1 | Q70WQ8 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-sepharose chromatography | Thermoproteus tenax |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + H2O | - |
Thermoproteus tenax | AMP + phosphoenolpyruvate + phosphate | - |
ir | |
ATP + pyruvate + H2O | - |
Thermoproteus tenax Kra1 | AMP + phosphoenolpyruvate + phosphate | - |
ir | |
additional information | neither GTP, CTP, ITP nor UTP is active as phosphoryl donor | Thermoproteus tenax | ? | - |
? | |
additional information | neither GTP, CTP, ITP nor UTP is active as phosphoryl donor | Thermoproteus tenax Kra1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | native enzyme, gel filtration | Thermoproteus tenax |
Synonyms | Comment | Organism |
---|---|---|
PEP synthetase | - |
Thermoproteus tenax |
PEPS | - |
Thermoproteus tenax |
phosphoenolpyruvate synthetase | - |
Thermoproteus tenax |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.683 | - |
pyruvate | in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C | Thermoproteus tenax |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Thermoproteus tenax |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
AMP | in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C | Thermoproteus tenax | |
0.6 | - |
2-oxoglutarate | in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C | Thermoproteus tenax | |
2.6 | - |
ADP | in 100 mM Tris/HCl, pH 7.0, in the presence of 20 mM beta-mercaptoethanol, 6 mM pyruvate, 10 mM ATP and 10 mM MgCl2, at 70°C | Thermoproteus tenax |