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Literature summary for 2.7.9.1 extracted from
- Purification, molecular, and catalytic properties of pyruvate phosphate dikinase from the maize leaf (1973), Biochemistry, 12, 2862-2868.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| method description | Zea mays |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
ATP | pH 7.5, 22ºC, phosphoenolpyruvate formation | Zea mays |  |
| 0.04 | - |
diphosphate | pH 7.5, 22ºC, pyruvate formation | Zea mays | |
| 0.14 | - |
phosphoenolpyruvate | pH 7.5, 22ºC, pyruvate formation | Zea mays | |
| 0.25 | - |
pyruvate | pH 7.5, 22ºC, phosphoenolpyruvate formation | Zea mays | |
| 1.5 | - |
phosphate | pH 7.5, 22ºC, phosphoenolpyruvate formation | Zea mays | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | free Mg2+ is required to stabilize the oligomeric structure of the enzyme | Zea mays | |
| NH4+ | at saturated concentration of NH4Cl pyruvate formation activity is increased 20fold | Zea mays | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 94000 | - |
4 * 94000, SDS-PAGE | Zea mays |
| 370000 | - |
gel filtration on Sepharose 6B | Zea mays |
| 387000 | - |
sedimentation analysis | Zea mays |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Zea mays | involved in C4 dicarboxylic acid pathway in plant | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + pyruvate + phosphate | - |
Zea mays | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
| additional information | involved in C4 dicarboxylic acid pathway in plant | Zea mays | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 94000, SDS-PAGE | Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Zea mays |
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