Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thiols | required for activity in solution | Zea mays |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | mediates a rapid but reversible inactivation in presence of a thiol | Arundinaria sp. | |
ADP | mediates a rapid but reversible inactivation in presence of a thiol | Zea mays | |
AMP | - |
Arundinaria sp. | |
AMP | - |
Zea mays | |
gamma(p-Arsenophenyl)-n-butyrate | - |
Arundinaria sp. | |
gamma(p-Arsenophenyl)-n-butyrate | - |
Zea mays | |
p-chloromercuribenzoate | - |
Arundinaria sp. | |
p-chloromercuribenzoate | - |
Zea mays | |
p-hydroxymercuribenzoate | - |
Zea mays | |
phosphate | - |
Arundinaria sp. | |
phosphate | - |
Zea mays | |
pyruvate | - |
Arundinaria sp. | |
pyruvate | - |
Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
AMP | pH 6.8, 25ºC | Arundinaria sp. | |
0.009 | - |
ATP | pH 8.3, 30ºC | Zea mays | |
0.04 | - |
diphosphate | - |
Zea mays | |
0.04 | - |
diphosphate | - |
Arundinaria sp. | |
0.09 | - |
ATP | pH 8.3 | Zea mays | |
0.11 | - |
pyruvate | pH 8.3, 30ºC | Zea mays | |
0.11 | - |
pyruvate | pH 8.3, 30ºC | Arundinaria sp. | |
0.5 | - |
phosphate | pH 8.3, 30ºC | Zea mays |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Zea mays | |
Mn2+ | not activation | Zea mays | |
NH4+ | - |
Zea mays |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
94000 | - |
4 * 94000, SDS-PAGE | Zea mays |
387000 | - |
sedimentation analysis | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arundinaria sp. | - |
- |
- |
Zea mays | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial, using Sephadex-G200 and Hypatite C chromatography | Zea mays |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate | sequential mechanism for the addition of ATP and phosphate and a ping-pong mechanism for the addition of pyruvate and release of phosphoenolpyruvate | Zea mays | |
ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate | sequential mechanism for the addition of ATP and phosphate and a ping-pong mechanism for the addition of pyruvate and release of phosphoenolpyruvate | Arundinaria sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
leaf | - |
Arundinaria sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.2 | - |
measured at 30ºC | Zea mays |
Storage Stability | Organism |
---|---|
0ºC, as a precipitate in a 66% saturated solution of (NH4)2SO4 | Zea mays |
0ºC, as a precipitate in a 66% saturated solution of (NH4)2SO4 | Arundinaria sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + arsenate | - |
Zea mays | ? | - |
ir | |
ATP + pyruvate + arsenate | - |
Arundinaria sp. | ? | - |
ir | |
ATP + pyruvate + phosphate | - |
Arundinaria sp. | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
ATP + pyruvate + phosphate | activity with UTP, GTP and CTP is 1-3% of the activity with ATP | Zea mays | AMP + phosphoenolpyruvate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 94000, SDS-PAGE | Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
rapid inactivation, activity is recovered by re-warming to 20-30ºC | Zea mays |
- |
- |
rapid inactivation, activity is recovered by re-warming to 20-30ºC | Arundinaria sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
phosphoenolpyruvate formation | Arundinaria sp. |