General Stability | Organism |
---|---|
freezing and thawing inactivates | [Clostridium] symbiosum |
sensitive to dilution, particularly at concentrations below 0.3 mg/ml, stable to freezing and thawing | Propionibacterium freudenreichii subsp. shermanii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5-dithiobis(2-nitrobenzoic acid) | - |
[Clostridium] symbiosum | |
5,5-dithiobis(2-nitrobenzoic acid) | - |
Propionibacterium freudenreichii subsp. shermanii | |
alpha-beta-methylene ATP | competitive to ATP | [Clostridium] symbiosum | |
alpha-beta-methylene ATP | competitive to ATP | Propionibacterium freudenreichii subsp. shermanii | |
beta-gamma-methylene ATP | competitive to ATP | [Clostridium] symbiosum | |
beta-gamma-methylene ATP | competitive to ATP | Propionibacterium freudenreichii subsp. shermanii | |
Bromopyruvate | competitive to phosphoenolpyruvate | [Clostridium] symbiosum | |
Bromopyruvate | competitive to phosphoenolpyruvate | Propionibacterium freudenreichii subsp. shermanii | |
Ca2+ | - |
[Clostridium] symbiosum | |
Ca2+ | competitive to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
CTP | - |
[Clostridium] symbiosum | |
CTP | - |
Propionibacterium freudenreichii subsp. shermanii | |
dATP | - |
[Clostridium] symbiosum | |
dATP | - |
Propionibacterium freudenreichii subsp. shermanii | |
Fe2+ | competitive to Mg2+ | Propionibacterium freudenreichii subsp. shermanii | |
GMP | competitive to ATP | Propionibacterium freudenreichii subsp. shermanii | |
GTP | competitive to ATP | Propionibacterium freudenreichii subsp. shermanii | |
iodoacetate | - |
[Clostridium] symbiosum | |
iodoacetate | - |
Propionibacterium freudenreichii subsp. shermanii | |
ITP | - |
[Clostridium] symbiosum | |
ITP | - |
Propionibacterium freudenreichii subsp. shermanii | |
Methylene diphosphonate | competitive to diphosphate | [Clostridium] symbiosum | |
Methylene diphosphonate | competitive to diphosphate | Propionibacterium freudenreichii subsp. shermanii | |
additional information | not inhibited by iodoacetamide | [Clostridium] symbiosum | |
additional information | not inhibited by iodoacetamide | Propionibacterium freudenreichii subsp. shermanii | |
N-ethylmaleimide | - |
[Clostridium] symbiosum | |
N-ethylmaleimide | - |
Propionibacterium freudenreichii subsp. shermanii | |
p-hydroxymercuribenzoate | - |
[Clostridium] symbiosum | |
p-hydroxymercuribenzoate | - |
Propionibacterium freudenreichii subsp. shermanii | |
Phosphoglycolate | competitive to phosphoenolpyruvate | [Clostridium] symbiosum | |
Sulfhydryl agents | - |
[Clostridium] symbiosum | |
Sulfhydryl agents | - |
Propionibacterium freudenreichii subsp. shermanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
AMP | pH 6.8, 25ºC | Propionibacterium freudenreichii subsp. shermanii | |
0.004 | - |
ATP | pH 6.8, 25ºC | Propionibacterium freudenreichii subsp. shermanii | |
0.015 | - |
AMP | pH 6.7, 25ºC | [Clostridium] symbiosum | |
0.05 | - |
ATP | pH 6.7, 25ºC | [Clostridium] symbiosum | |
0.08 | - |
diphosphate | pH 6.7, 25ºC | [Clostridium] symbiosum | |
0.1 | - |
pyruvate | pH 6.8, 25ºC | Propionibacterium freudenreichii subsp. shermanii | |
0.1 | - |
pyruvate | pH 6.7, 25ºC | [Clostridium] symbiosum | |
0.1 | - |
phosphoenolpyruvate | pH 6.7, 25ºC | [Clostridium] symbiosum | |
0.8 | - |
phosphate | pH 6.7, 25ºC | [Clostridium] symbiosum | |
1 | - |
phosphate | pH 6.8, 25ºC | Propionibacterium freudenreichii subsp. shermanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
[Clostridium] symbiosum | |
K+ | - |
[Clostridium] symbiosum | |
Mg2+ | - |
[Clostridium] symbiosum | |
Mn2+ | - |
[Clostridium] symbiosum | |
NH4+ | - |
[Clostridium] symbiosum | |
Rb+ | 25% of the activity relative to NH4+ | [Clostridium] symbiosum | |
Tl+ | 94% of the activity relative to NH4+ | [Clostridium] symbiosum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
2 * 75000, SDS-PAGE | [Clostridium] symbiosum |
150000 | - |
gel filtration | Propionibacterium freudenreichii subsp. shermanii |
150000 | 160000 | equilibrium sedimentation and gel filtration | [Clostridium] symbiosum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
[Clostridium] symbiosum | - |
ATCC 14940 | - |
Purification (Comment) | Organism |
---|---|
partial, using ammonium sulfate precipitation and chromatography on DEAE-cellulose and Sephadex G-200 | Propionibacterium freudenreichii subsp. shermanii |
partial, using ammonium sulfate precipitation, and chromatography on DEAE-cellulose and Bio-Gel | [Clostridium] symbiosum |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate | mechanism | Propionibacterium freudenreichii subsp. shermanii | |
ATP + pyruvate + phosphate = AMP + phosphoenolpyruvate + diphosphate | mechanism | [Clostridium] symbiosum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.1 | - |
- |
Propionibacterium freudenreichii subsp. shermanii |
35 | - |
- |
[Clostridium] symbiosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | specificity is strictly restricted to adenine nucleotides | Propionibacterium freudenreichii subsp. shermanii | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
ATP + pyruvate + phosphate | specificity is strictly restricted to adenine nucleotides | [Clostridium] symbiosum | AMP + phosphoenolpyruvate + diphosphate | - |
? | |
ATP + pyruvate + phosphate | dAMP can replace ATP but with 20% of the activity | [Clostridium] symbiosum | AMP + phosphoenolpyruvate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 75000, SDS-PAGE | [Clostridium] symbiosum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Propionibacterium freudenreichii subsp. shermanii |
25 | - |
assay at | [Clostridium] symbiosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.4 | 6.7 | pyruvate formation | [Clostridium] symbiosum |
6.5 | 7.5 | phosphoenolpyruvate formation | Propionibacterium freudenreichii subsp. shermanii |
6.8 | - |
pyruvate formation | Propionibacterium freudenreichii subsp. shermanii |
7 | 7.8 | phosphoenolpyruvate formation | [Clostridium] symbiosum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 6.7 | - |
Propionibacterium freudenreichii subsp. shermanii |