BRENDA - Enzyme Database show
show all sequences of 2.7.8.8

Phosphatidylserine synthase controls cell elongation especially in the uppermost internode in rice by regulation of exocytosis

Ma, J.; Cheng, Z.; Chen, J.; Shen, J.; Zhang, B.; Ren, Y.; Ding, Y.; Zhou, Y.; Zhang, H.; Zhou, K.; Wang, J.L.; Lei, C.; Zhang, X.; Guo, X.; Gao, H.; Bao, Y.; Wan, J.M.; PLoS ONE 11, e0153119 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
transformation of OsPSS-1-GFP in Arabidopsis thaliana protoplasts, subcellular localization in endoplasmic reticulum, plasma membrane, and trans-Golgi network/early endosomes
Oryza sativa Japonica Group
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Oryza sativa Japonica Group
5783
-
endosome
early endosomes
Oryza sativa Japonica Group
5768
-
Golgi apparatus
-
Oryza sativa Japonica Group
5794
-
additional information
OsPSS-1 localizes to organelles associated with exocytosis
Oryza sativa Japonica Group
-
-
plasma membrane
-
Oryza sativa Japonica Group
5886
-
trans-Golgi network
-
Oryza sativa Japonica Group
5802
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CDP-diacylglycerol + L-serine
Oryza sativa Japonica Group
-
CMP + (3-sn-phosphatidyl)-L-serine
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Oryza sativa Japonica Group
Q0JR55
cv. Kitaake
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
culm
-
Oryza sativa Japonica Group
-
internode
especially the uppermost; isozyme OsPSS-1 gene is expressed predominantly in elongating cells
Oryza sativa Japonica Group
-
leaf
-
Oryza sativa Japonica Group
-
additional information
quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses of wild-type plants reveals that OsPSS-1 is expressed in various organs, including roots, culms, and leaves, with the highest expression in panicles
Oryza sativa Japonica Group
-
panicle
-
Oryza sativa Japonica Group
-
root
-
Oryza sativa Japonica Group
-
seedling
-
Oryza sativa Japonica Group
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CDP-diacylglycerol + L-serine
-
739446
Oryza sativa Japonica Group
CMP + (3-sn-phosphatidyl)-L-serine
-
-
-
?
Cloned(Commentary) (protein specific)
Commentary
Organism
transformation of OsPSS-1-GFP in Arabidopsis thaliana protoplasts, subcellular localization in endoplasmic reticulum, plasma membrane, and trans-Golgi network/early endosomes
Oryza sativa Japonica Group
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
endoplasmic reticulum
-
Oryza sativa Japonica Group
5783
-
endosome
early endosomes
Oryza sativa Japonica Group
5768
-
Golgi apparatus
-
Oryza sativa Japonica Group
5794
-
additional information
OsPSS-1 localizes to organelles associated with exocytosis
Oryza sativa Japonica Group
-
-
plasma membrane
-
Oryza sativa Japonica Group
5886
-
trans-Golgi network
-
Oryza sativa Japonica Group
5802
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CDP-diacylglycerol + L-serine
Oryza sativa Japonica Group
-
CMP + (3-sn-phosphatidyl)-L-serine
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
culm
-
Oryza sativa Japonica Group
-
internode
especially the uppermost; isozyme OsPSS-1 gene is expressed predominantly in elongating cells
Oryza sativa Japonica Group
-
leaf
-
Oryza sativa Japonica Group
-
additional information
quantitative reverse transcription polymerase chain reaction (qRT-PCR) analyses of wild-type plants reveals that OsPSS-1 is expressed in various organs, including roots, culms, and leaves, with the highest expression in panicles
Oryza sativa Japonica Group
-
panicle
-
Oryza sativa Japonica Group
-
root
-
Oryza sativa Japonica Group
-
seedling
-
Oryza sativa Japonica Group
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CDP-diacylglycerol + L-serine
-
739446
Oryza sativa Japonica Group
CMP + (3-sn-phosphatidyl)-L-serine
-
-
-
?
General Information
General Information
Commentary
Organism
malfunction
mutation of OsPSS-1 leads to compromised delivery of CESA4 and secGFP towards the cell surface, resulting in weakened intercellular adhesion and disorganized cell arrangement in parenchyma. The Dwarf phenotype of shortened uppermost internode 1 (sui1) is caused by mutations in phosphatidylserine synthase. The phenotype of sui1-4 is caused largely by the reduction in cellulose contents in the whole plant and detrimental delivery of pectins in the uppermost internode. sui1-4 plants exhibit compromised secretion. The mutants show reduced length of both panicles and internodes, especially the uppermost internode, accompanied with reduced fertility, decreased grain size and slightly increased tiller number, defective pectin secretion, detailed overview. A large amount of OsCESA4 remained in the cytoplasm in the mutant, most likely due to failure in delivery to the plasma membrane
Oryza sativa Japonica Group
physiological function
the primary role of PSS enzymes is (3-sn-phosphatidyl)-L-serine biosynthesis, and isozyme PPS1 regulates post-Golgi vesicle secretion to intercellular spaces, the enzyme function is associated with exocytosis. Isozyme PSS1 plays a potential role in mediating cell expansion by regulating secretion of cell wall components
Oryza sativa Japonica Group
General Information (protein specific)
General Information
Commentary
Organism
malfunction
mutation of OsPSS-1 leads to compromised delivery of CESA4 and secGFP towards the cell surface, resulting in weakened intercellular adhesion and disorganized cell arrangement in parenchyma. The Dwarf phenotype of shortened uppermost internode 1 (sui1) is caused by mutations in phosphatidylserine synthase. The phenotype of sui1-4 is caused largely by the reduction in cellulose contents in the whole plant and detrimental delivery of pectins in the uppermost internode. sui1-4 plants exhibit compromised secretion. The mutants show reduced length of both panicles and internodes, especially the uppermost internode, accompanied with reduced fertility, decreased grain size and slightly increased tiller number, defective pectin secretion, detailed overview. A large amount of OsCESA4 remained in the cytoplasm in the mutant, most likely due to failure in delivery to the plasma membrane
Oryza sativa Japonica Group
physiological function
the primary role of PSS enzymes is (3-sn-phosphatidyl)-L-serine biosynthesis, and isozyme PPS1 regulates post-Golgi vesicle secretion to intercellular spaces, the enzyme function is associated with exocytosis. Isozyme PSS1 plays a potential role in mediating cell expansion by regulating secretion of cell wall components
Oryza sativa Japonica Group
Other publictions for EC 2.7.8.8
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
739446
Ma
Phosphatidylserine synthase co ...
Oryza sativa Japonica Group
PLoS ONE
11
e0153119
2016
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723504
Yin
SUI-family genes encode phosph ...
Oryza sativa
Planta
237
15-27
2013
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738817
Liu
Phosphatidylserine synthase 1 ...
Arabidopsis thaliana
J. Integr. Plant Biol.
55
682-695
2013
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722905
Morita
Enzymatic measurement of phosp ...
Homo sapiens
J. Lipid Res.
53
325-330
2012
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723425
Yamaoka
Phosphatidylserine synthase1 i ...
Arabidopsis thaliana
Plant J.
67
648-661
2011
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705791
Chen
Phosphatidylserine synthase an ...
Candida albicans
Mol. Microbiol.
75
1112-1132
2010
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704125
Zhang
Expression, purification, and ...
Escherichia coli
J. Agric. Food Chem.
57
122-126
2009
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661745
Linde
Lipid dependence and activity ...
Escherichia coli
FEBS Lett.
575
77-80
2004
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692823
Morii
CDP-2,3-di-O-geranylgeranyl-sn ...
Escherichia coli
J. Bacteriol.
185
1181-1189
2003
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643796
Rilfors
Reconstituted phosphatidylseri ...
Escherichia coli
Biochim. Biophys. Acta
1438
281-294
1999
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643797
Matsumoto
Phosphatidylserine synthase fr ...
Bacillus subtilis, Escherichia coli
Biochim. Biophys. Acta
1348
214-227
1997
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643779
Dowhan
Phosphatidylserine synthase fr ...
Escherichia coli
Methods Enzymol.
209
287-298
1992
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643789
Raetz
Phospholipids chiral at phosph ...
Escherichia coli
Biochemistry
26
4022-4027
1987
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643791
Louie
Substrate-induced membrane ass ...
Escherichia coli
J. Bacteriol.
165
805-812
1986
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643778
Dowhan
-
Phosphatidylserine synthase fr ...
Escherichia coli
Methods Enzymol.
71
561-571
1981
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643782
Carman
Phosphatidylserine synthase fr ...
Escherichia coli
J. Biol. Chem.
254
8391-8397
1979
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643776
Larson
Ribosomal-associated phosphati ...
Escherichia coli
Biochemistry
15
5212-5218
1976
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643777
Raetz
-
Partial purification and prope ...
Escherichia coli
J. Biol. Chem.
249
5038-5045
1974
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643784
Ishinaga
Effects of phospholipids on so ...
Escherichia coli
FEBS Lett.
49
201-202
1974
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643781
Raetz
The association of phosphatidy ...
Escherichia coli
J. Biol. Chem.
247
2008-2014
1972
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2
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1
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