BRENDA - Enzyme Database
show all sequences of 2.7.8.44

The TagB protein in Bacillus subtilis 168 is an intracellular peripheral membrane protein that can incorporate glycerol phosphate onto a membrane-bound acceptor in vitro

Bhavsar, A.P.; Truant, R.; Brown, E.D.; J. Biol. Chem. 280, 36691-36700 (2005)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 cells
Bacillus subtilis
Engineering
Amino acid exchange
Commentary
Organism
H126A
the mutant shows 75% less activity relative to the wild type enzyme
Bacillus subtilis
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasmic membrane
-
Bacillus subtilis
-
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Bacillus subtilis
-
CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
-
?
CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Bacillus subtilis 168
-
CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
P27621
-
-
Bacillus subtilis 168
P27621
-
-
Purification (Commentary)
Commentary
Organism
HiTrap column chromatography and Superdex 200 gel filtration
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
740678
Bacillus subtilis
CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
-
-
?
CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
740678
Bacillus subtilis 168
CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 47079, calculated from amino acid sequence
Bacillus subtilis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 cells
Bacillus subtilis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
H126A
the mutant shows 75% less activity relative to the wild type enzyme
Bacillus subtilis
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytoplasmic membrane
-
Bacillus subtilis
-
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Bacillus subtilis
-
CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
-
?
CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
Bacillus subtilis 168
-
CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
HiTrap column chromatography and Superdex 200 gel filtration
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
740678
Bacillus subtilis
CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
-
-
?
CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
740678
Bacillus subtilis 168
CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 47079, calculated from amino acid sequence
Bacillus subtilis
Other publictions for EC 2.7.8.44
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
740622
Chan
Staphylococcus aureus mutants ...
Staphylococcus aureus, Staphylococcus aureus RN4220
J. Bacteriol.
195
4650-4659
2013
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20
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1
1
-
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-
740296
Brown
a revised pathway proposed for ...
Staphylococcus aureus, Staphylococcus aureus NCTC 8325
Chem. Biol.
15
12-21
2008
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2
-
2
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2
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2
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2
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740611
Formstone
Localization and interactions ...
Bacillus subtilis 168, Bacillus subtilis
J. Bacteriol.
190
1812-1821
2008
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1
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2
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5
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2
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1
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2
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2
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740610
Bhavsar
The Amino terminus of Bacillus ...
Bacillus subtilis 168, Bacillus subtilis
J. Bacteriol.
189
6816-6823
2007
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-
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1
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2
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5
-
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2
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1
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2
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2
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-
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1
1
-
-
-
740607
DElia
Lesions in teichoic acid biosy ...
Staphylococcus aureus, Staphylococcus aureus SA178RI
J. Bacteriol.
188
4183-4189
2006
-
-
-
-
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2
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-
-
740678
Bhavsar
The TagB protein in Bacillus s ...
Bacillus subtilis, Bacillus subtilis 168
J. Biol. Chem.
280
36691-36700
2005
-
-
1
-
1
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-
-
1
-
-
2
-
7
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1
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2
1
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1
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1
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1
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2
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1
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2
1
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741467
Prayitno
-
The effects of growth conditio ...
Bacillus subtilis, Bacillus subtilis 168
World J. Microbiol. Biotechnol.
13
207-217
1997
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1
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2
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5
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2
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1
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2
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