Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | no activitation by binding to DNa required | Vibrio cholerae O1 |
Crystallization (Comment) | Organism |
---|---|
wild-type and mutant D131N/D133N. Catalytic residues are D131 and D133 in strand beta1, and D193 in strand beta5, and the G[G/S]xx motif is defined by residues G113-S114-F115-Q116 in a well-defined loop linking helix alpha5 and strand beta1 | Vibrio cholerae O1 |
Protein Variants | Comment | Organism |
---|---|---|
D131N | mutation in conserved acidic residue, loss of activity | Vibrio cholerae O1 |
D131N/D133N | mutation in conserved acidic residues, loss of activity | Vibrio cholerae O1 |
D133N | mutation in conserved acidic residue, loss of activity | Vibrio cholerae O1 |
D193N | mutation in conserved acidic residue, loss of activity | Vibrio cholerae O1 |
K287A | mutation in residue that participates in the interaction with diphosphate, impairs the ability to catalyze cyclic di-GMP formation | Vibrio cholerae O1 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GTP | Vibrio cholerae O1 | - |
2 diphosphate + cyclic Ap(3'-5')Gp | - |
? | |
ATP + GTP | Vibrio cholerae O1 ATCC 39315 | - |
2 diphosphate + cyclic Ap(3'-5')Gp | - |
? | |
GTP + ATP | Vibrio cholerae O1 | - |
2 diphosphate + cyclic Gp(3'-5')A | in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated | ? | |
GTP + ATP | Vibrio cholerae O1 ATCC 39315 | - |
2 diphosphate + cyclic Gp(3'-5')A | in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated | ? | |
GTP + GTP | Vibrio cholerae O1 | - |
2 diphosphate + cyclic Gp(3'-5')G | - |
? | |
GTP + GTP | Vibrio cholerae O1 ATCC 39315 | - |
2 diphosphate + cyclic Gp(3'-5')G | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio cholerae O1 | Q9KVG7 | - |
- |
Vibrio cholerae O1 ATCC 39315 | Q9KVG7 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + GTP | - |
Vibrio cholerae O1 | 2 diphosphate + cyclic Ap(3'-5')Gp | - |
? | |
ATP + GTP | - |
Vibrio cholerae O1 | 2 diphosphate + cyclic Ap(3'-5')Gp | a mixture of GTP and ATP triggers the synthesis of cGAMP as the dominant product, with little c-di-AMP and c-di-GMP generated | ? | |
ATP + GTP | - |
Vibrio cholerae O1 ATCC 39315 | 2 diphosphate + cyclic Ap(3'-5')Gp | - |
? | |
ATP + GTP | - |
Vibrio cholerae O1 ATCC 39315 | 2 diphosphate + cyclic Ap(3'-5')Gp | a mixture of GTP and ATP triggers the synthesis of cGAMP as the dominant product, with little c-di-AMP and c-di-GMP generated | ? | |
GTP + ATP | - |
Vibrio cholerae O1 | 2 diphosphate + cyclic Gp(3'-5')A | in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated | ? | |
GTP + ATP | - |
Vibrio cholerae O1 ATCC 39315 | 2 diphosphate + cyclic Gp(3'-5')A | in presence of a mixture of GTP and ATP cGAMP is the dominant product, with little cyclic di-AMP and cyclic di-GMP generated | ? | |
GTP + GTP | - |
Vibrio cholerae O1 | 2 diphosphate + cyclic Gp(3'-5')G | - |
? | |
GTP + GTP | - |
Vibrio cholerae O1 ATCC 39315 | 2 diphosphate + cyclic Gp(3'-5')G | - |
? |