Literature summary for 2.7.7.B22 extracted from

Kia, A.; Gloeckner, C.; Osothprarop, T.; Gormley, N.; Bomati, E.; Stephenson, M.; Goryshin, I.; He, M.M.
Improved genome sequencing using an engineered transposase (2017), BMC Biotechnol., 17, 006 .

Search for more literature for 2.7.7.B22

Application

Application	Comment	Organism
analysis	mutant K212R/P214R/G251R/A338V, i.e. Tn5-059, displays a lowered GC insertion bias. Tn5-059 reduces AT dropout and increases uniformity of genome coverage in both bacterial genomes and human genome. Tn5-059 generates a higher library diversion when compared to Nextera v2 for human exomes. When used for human exomes, Tn5-059 delivers consistent library insert size over a range of input DNA, allowing up to a tenfold variance from the 50 ng input recommendation	Escherichia coli

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Escherichia coli

Protein Variants

Protein Variants	Comment	Organism
K212R/P214R/G251R/A338V	i.e. Tn5-059, mutant displays a lowered GC insertion bias. Tn5-059 reduces AT dropout and increases uniformity of genome coverage in both bacterial genomes and human genome	Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	Q46731	-	-

Synonyms

Synonyms	Comment	Organism
Tn5 transposase	-	Escherichia coli
TnpA	-	Escherichia coli

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)