Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the sleeping beauty transposon DNA contains the gene to be inserted into the target DNA flanked by inverted terminal repeats (IR-DRleft and IR-DRright). Each inverted terminal repeat contains two, inner and outer, direct repeats (DRs). The DRs represent binding sites for the transposase enzyme. Sleeping beauty, SB, appears to be the most random in preferences for integration sites, requiring only a TA dinucleotide basepair. Binding mechanism, detailed overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the sleeping beauty transposon DNA contains the gene to be inserted into the target DNA flanked by inverted terminal repeats (IR-DRleft and IR-DRright). Each inverted terminal repeat contains two, inner and outer, direct repeats (DRs). The DRs represent binding sites for the transposase enzyme. Sleeping beauty, SB, appears to be the most random in preferences for integration sites, requiring only a TA dinucleotide basepair. Binding mechanism, detailed overview | Homo sapiens | ? | - |
? | |
additional information | interaction analysis of full-length enzyme and of PAI subdomain with transposon DNA, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | domain structure of SB transposase enzyme, and solution conformation of full-length DNA-binding domain of SB transposase, [15N,1H]-HSQC spectra, overview. The DNA-binding domain is predicted to have two subdomains, PAI and RED, containing three alpha-helices. Residues 97-123 represent a nuclear localization signal (NLS). The catalytic domain contains three conserved catalytic residues, the DDE motif. NMR solution structure of the PAI subdomain, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
SB transposase | - |
Homo sapiens |
sleeping beauty transposase | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the SB transposon is the most active DNA transposon in vertebrate animal cells | Homo sapiens |