Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.72 extracted from

  • Shi, P.Y.; Maizels, N.; Weiner, A.M.
    CCA addition by tRNA nucleotidyltransferase: polymerization without translocation? (1998), EMBO J., 17, 3197-3206.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.72

Inhibitors

Inhibitors Comment Organism Structure
ethylnitrosourea both CTP addition to tRNA-C and ATP addition to tRNA-CC are dramatically inhibited by alkylation of the same tRNA phosphates in the acceptor stem and TPsiC stem-loop Escherichia coli
ethylnitrosourea both CTP addition to tRNA-C and ATP addition to tRNA-CC are dramatically inhibited by alkylation of the same tRNA phosphates in the acceptor stem and TPsiC stem-loop Saccharolobus shibatae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the CCA-adding enzymes can use three different substrates: tRNAs lacking one, i.e. tRNA-CC, two, i.e. tRNA-C, or all three 3'-terminal nucleotides, tRNA. The CCA-adding enzyme recognizes primarily the top half tRNA minihelix ?
-
 ?
additional information Saccharolobus shibatae the CCA-adding enzymes can use three different substrates: tRNAs lacking one, i.e. tRNA-CC, two, i.e. tRNA-C, or all three 3'-terminal nucleotides, tRNA. The Sulfolobus shibatae CCA-adding enzyme forms a stable complex with tRNA. The CCA-adding enzyme recognizes primarily the top half tRNA minihelix ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Saccharolobus shibatae
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the CCA-adding enzymes can use three different substrates: tRNAs lacking one, i.e. tRNA-CC, two, i.e. tRNA-C, or all three 3'-terminal nucleotides, tRNA. The CCA-adding enzyme recognizes primarily the top half tRNA minihelix Escherichia coli ?
-
 ?
additional information the CCA-adding enzymes can use three different substrates: tRNAs lacking one, i.e. tRNA-CC, two, i.e. tRNA-C, or all three 3'-terminal nucleotides, tRNA. The Sulfolobus shibatae CCA-adding enzyme forms a stable complex with tRNA. The CCA-adding enzyme recognizes primarily the top half tRNA minihelix Saccharolobus shibatae ?
-
 ?
additional information the tRNA substrate must remain fixed on the enzyme surface during CA addition, tRNA-C cross-linked to the enzyme remains fully active for addition of CTP and ATP. The growing 3'-terminus of the tRNA progressively refolds to allow the solitary active site to reuse a single CTP binding site. The ATP binding site is then created collaboratively by the refolded CCterminus and the enzyme, and nucleotide addition ceases when the nucleotide binding pocket is full. The template for CCA addition is a dynamic ribonucleoprotein structure. Both CTP addition to tRNA-C and ATP addition to tRNA-CC are dramatically inhibited by alkylation of the same tRNA phosphates in the acceptor stem and TPsiC stem-loop Saccharolobus shibatae ?
-
 ?
additional information the tRNA substrate must remain fixed on the enzyme surface during CA addition. Both CTP addition to tRNA-C and ATP addition to tRNA-CC are dramatically inhibited by alkylation of the same tRNA phosphates in the acceptor stem and TPsiC stem-loop Escherichia coli ?
-
 ?
tRNAAsp with a 3' CC end + ATP usage of tRNAAsp of Bacillus subtilis as substrate, and 5'-labeled tRNA-C and 3'-labeled tRNA-CC alkylated by ethylnitrosourea Escherichia coli tRNAAsp with a 3' CCA end + diphosphate
-
 ?
tRNAAsp with a 3' CC end + ATP usage of tRNAAsp of Bacillus subtilis as substrate, and 5'-labeled tRNA-C and 3'-labeled tRNA-CC alkylated by ethylnitrosourea Saccharolobus shibatae tRNAAsp with a 3' CCA end + diphosphate
-
 ?
tRNAAsp with a 3' cytidine + CTP usage of tRNAAsp of Bacillus subtilis as substrate, and 5'-labeled tRNA-C and 3'-labeled tRNA-CC alkylated by ethylnitrosourea Escherichia coli tRNAAsp with a 3' CC end + diphosphate
-
 ?
tRNAAsp with a 3' cytidine + CTP usage of tRNAAsp of Bacillus subtilis as substrate, and 5'-labeled tRNA-C and 3'-labeled tRNA-CC alkylated by ethylnitrosourea Saccharolobus shibatae tRNAAsp with a 3' CC end + diphosphate
-
 ?

Synonyms

Synonyms Comment Organism
ATP(CTP):tRNA nucleotidyltransferase
-
 Escherichia coli
ATP(CTP):tRNA nucleotidyltransferase
-
 Saccharolobus shibatae
CCA adding enzyme
-
-
class I CCA adding enzyme
-
 Saccharolobus shibatae
class II CCA adding enzyme
-
 Escherichia coli
More the CCA-adding enzymes belong to the nucleotidyltransferase superfamily Escherichia coli
More the CCA-adding enzymes belong to the nucleotidyltransferase superfamily Saccharolobus shibatae
tRNA nucleotidyltransferase
-
 Escherichia coli
tRNA nucleotidyltransferase
-
 Saccharolobus shibatae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Escherichia coli
25
-
 assay at Saccharolobus shibatae