Literature summary for 2.7.7.7 extracted from

Vashishtha, A.K.; Konigsberg, W.H.
Effect of different divalent cations on the kinetics and fidelity of RB69 DNA polymerase (2016), Biochemistry, 55, 2661-2670 .

Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment)	Organism
-	Escherichia phage RB69

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	competes with Mg2+ for metal ion binding site(s)	Escherichia phage RB69
Co2+	can effectively replace Mg2+. When Mg2+ is replaced with Co2+, the efficiency of incorporation of dTMP opposite dA increases by 5-fold	Escherichia phage RB69
Mg2+	activates	Escherichia phage RB69
Mn2+	activates. When Mg2+ is replaced with Mn2+, the efficiency of incorporation of dTMP opposite dA increases by 3fold	Escherichia phage RB69
additional information	no activation by Fe2+, Ca2+, Zn2+, Cd2+, Sr2+, Ba2+, Cu2+, and Cr3+. Ca2+ concentration required to obtain the half-maximal incorporation rate under single-turnover conditions was 0.63 mM	Escherichia phage RB69
Ni2+	slight activation	Escherichia phage RB69

Organism

Organism	UniProt	Comment	Textmining
Escherichia phage RB69	Q38087	-	-

Purification (Commentary)

Purification (Comment)	Organism
overexpressed in Escherichia coli	Escherichia phage RB69

Synonyms

Synonyms	Comment	Organism
RB69 DNA Polymerase	-	Escherichia phage RB69
RB69pol	-	Escherichia phage RB69

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Release 2023.1 (January 2023)