Literature summary for 2.7.7.7 extracted from

Din, R.U.; Khan, M.I.; Jan, A.; Khan, S.A.; Ali, I.
A novel approach for high-level expression and purification of GST-fused highly thermostable Taq DNA polymerase in Escherichia coli (2020), Arch. Microbiol., 202, 1449-1458 .

Search for more literature for 2.7.7.7

Application

Application	Comment	Organism
molecular biology	the thermostable properties of the enzyme from Thermus aquaticus have contributed majorly to the specificity, automation, and efficacy of the polymerase chain reaction (PCR)	Thermus aquaticus

Cloned(Commentary)

Cloned (Comment)	Organism
high-level expression in Escherichia coli BL21. A 2500 base pair Taq DNA polymerase gene is colned into pGEX-4T-1 vector, containing a GSTtag, downstream of tac promoter and overexpressed it using isopropyl ß-d-1-thiogalactopyranoside (IPTG) as an inducer	Thermus aquaticus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
94000	-	SDS-PAGE	Thermus aquaticus

Organism

Organism	UniProt	Comment	Textmining
Thermus aquaticus	P19821	-	-

Purification (Commentary)

Purification (Comment)	Organism
the purified recombinant Taq DNA polymerase shows better performance in comparison to commercially available polymerases	Thermus aquaticus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	the purified recombinant Taq DNA polymerase shows better performance in comparison to commercially available polymerases	Thermus aquaticus

Synonyms

Synonyms	Comment	Organism
Taq DNA polymerase	-	Thermus aquaticus

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Release 2023.1 (January 2023)