Literature summary for 2.7.7.7 extracted from

Matsui, I.; Urushibata, Y.; Shen, Y.; Matsui, E.; Yokoyama, H.
Novel structure of an N-terminal domain that is crucial for the dimeric assembly and DNA-binding of an archaeal DNA polymerase D large subunit from Pyrococcus horikoshii (2011), FEBS Lett., 585, 452-458 .

Crystallization (Commentary)

Crystallization (Comment)	Organism
crystals are grown at 20°C according to the hanging-drop vapor-diffusion method. The N-terminal (1-300) domain structure of the large subunit was determined by X-ray crystallography, although about 50 N-terminal residues are disordered. The determined structure consists of nine alpha helices and three beta strands. The DNA-binding ability of the domain by is identified by SPR measurement. The N-terminal (1-100) region plays crucial roles in the folding of the large subunit dimer by connecting the about 50 N-terminal residues with their own catalytic region (792-1163)	Pyrococcus horikoshii

Crystallization (Comment)

Organism

crystals are grown at 20°C according to the hanging-drop vapor-diffusion method. The N-terminal (1-300) domain structure of the large subunit was determined by X-ray crystallography, although about 50 N-terminal residues are disordered. The determined structure consists of nine alpha helices and three beta strands. The DNA-binding ability of the domain by is identified by SPR measurement. The N-terminal (1-100) region plays crucial roles in the folding of the large subunit dimer by connecting the about 50 N-terminal residues with their own catalytic region (792-1163)

Pyrococcus horikoshii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
420000	-	-	Pyrococcus horikoshii

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus horikoshii	O57863 and O57861	O57863: DNA polymerase II small subunit, gene name polB, ordered locus name: PH0123, O57861: DNA polymerase II large subunit, gene name polC, ordered locus name: PH0121	-
Pyrococcus horikoshii OT-3	O57863 and O57861	O57863: DNA polymerase II small subunit, gene name polB, ordered locus name: PH0123, O57861: DNA polymerase II large subunit, gene name polC, ordered locus name: PH0121	-

Subunits

Subunits	Comment	Organism
heterotetramer	heterotetramer consisting of two small subunits (locus name: PH0123) and two large subunits (locus name: PH0121). The large subunits is the catalytic subunit of DNA polymerase, while the small subunits is the catalytic subunit of Mre11-like 3'-5' exonuclease	Pyrococcus horikoshii

Synonyms

Synonyms	Comment	Organism
hoPolD	-	Pyrococcus horikoshii
PH0121	locus name of DNA polymerase II large subunit	Pyrococcus horikoshii
PH0123	locus name of DNA polymerase II small subunit	Pyrococcus horikoshii
polD	-	Pyrococcus horikoshii