Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus fumicolans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | increase in KCl concentration from 10 to 75 mM results in a 10fold increase in polymerase activity | Thermococcus fumicolans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89000 | - |
protein band possibly corresponding to mature DNA polymerase, SDS-PAGE | Thermococcus fumicolans |
170000 | - |
protein precursor containing the DNA polymerase with both inteins, SDS-PAGE | Thermococcus fumicolans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus fumicolans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus fumicolans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Thermococcus fumicolans | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase I | - |
Thermococcus fumicolans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
72 | - |
- |
Thermococcus fumicolans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
92 | - |
retains 50% of its activity after 7 h | Thermococcus fumicolans |
95 | - |
retains 50% of its activity after 3.3 h | Thermococcus fumicolans |
100 | - |
retains 50% of its activity after 2 h | Thermococcus fumicolans |