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Literature summary for 2.7.7.65 extracted from

  • Zhu, B.; Liu, C.; Liu, S.; Cong, H.; Chen, Y.; Gu, L.; Ma, L.Z.
    Membrane association of SadC enhances its diguanylate cyclase activity to control exopolysaccharides synthesis and biofilm formation in Pseudomonas aeruginosa (2016), Environ. Microbiol., 18, 3440-3452 .
    View publication on PubMed
Search for more literature for 2.7.7.65

Activating Compound

Activating Compound Comment Organism Structure
additional information membrane association of SadC promotes its DGC activity by affecting the formation of active DGC oligomers Pseudomonas aeruginosa

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant truncated enzymes SadC323-487 and SadC300-487, X-ray diffraction structure determination and analysis at 1.8 A and 2.8 A resolution, respectively. The structure contains ine Mg2+, molecular replacement structure analysis Pseudomonas aeruginosa

Protein Variants

Protein Variants Comment Organism
additional information construction of an N-terminally truncated SadC containing amino acid residue 300-487, which included the entire GGDEF domain and 23 amino acid residues at the N-terminus to link GGDEF domain with the transmembrane domains. Recombinant strain PAO1/pSadC300-487 fails to promote bacterial cell aggregation even if its expression is induced by 2% arabinose. Moreover, SadC300-487 does not significantly enhance the biofilm formation of PAO1 although SadC300-487 can increase Psl production to a level close to the full-length SadC when induced by 2% arabinose. The crystal structure of SadC323-487 indicates that this truncated SadC is a monomer in the asymmetric unit. The structure of SadC323-487 contains five beta-sheets sandwiched by five alpha-helices, which is a typical GGDEF structure. SadC300-487 can catalyze the synthesis of c-di-GMP in vitro, but SadC323-487 cannot, structure-function analysis, overview Pseudomonas aeruginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
inner membrane SadC is an inner membrane protein, which is predicted to have five transmembrane domains at N-terminal and a GGDEF domain at C-terminal. Membrane association of SadC promotes its DGC activity by affecting the formation of active DGC oligomers Pseudomonas aeruginosa
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 GTP Pseudomonas aeruginosa
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 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa ATCC 15692
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 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa 1C
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa PRS 101
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa DSM 22644
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa CIP 104116
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa LMG 12228
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP Pseudomonas aeruginosa JCM 14847
-
 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa Q9HW69
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Pseudomonas aeruginosa 1C Q9HW69
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Pseudomonas aeruginosa ATCC 15692 Q9HW69
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Pseudomonas aeruginosa CIP 104116 Q9HW69
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Pseudomonas aeruginosa DSM 22644 Q9HW69
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Pseudomonas aeruginosa JCM 14847 Q9HW69
-
-
Pseudomonas aeruginosa LMG 12228 Q9HW69
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-
Pseudomonas aeruginosa PRS 101 Q9HW69
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-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 GTP
-
 Pseudomonas aeruginosa 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa ATCC 15692 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa 1C 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa PRS 101 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa DSM 22644 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa CIP 104116 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa LMG 12228 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?
2 GTP
-
 Pseudomonas aeruginosa JCM 14847 2 diphosphate + cyclic di-3',5'-guanylate
-
 ?

Subunits

Subunits Comment Organism
More the GGDEF domain of SadC has a typical GGDEF structure and the alpha-helix connecting the transmembrane domains with SadC GGDEF domain is essential for SadC to form DGC oligomers. Membrane association of SadC promotes its DGC activity by affecting the formation of active DGC oligomers. The alpha-helix of amino acid residues 300-322 is necessary for SadC300-487 to form active hexamers, hexameric and monomeric structures. The structure of SadC323-487 contains five beta-sheets sandwiched by five alpha-helices, which is a typical GGDEF structure. Enzyme mutants structure-function analysis, overview Pseudomonas aeruginosa

Synonyms

Synonyms Comment Organism
GGDEF domain-containing protein UniProt Pseudomonas aeruginosa
sadC
-
 Pseudomonas aeruginosa

General Information

General Information Comment Organism
malfunction overexpression of SadC in Pseudomonas aeruginosa strain PAO1 totally inhibits swimming motility and significantly enhances the production of exopolysaccharide Psl. SadC lacking transmembrane domains (SadC300-487) cannot localize on cytoplasmic membrane and form cluster, loses the ability to inhibit the swimming and twitching motility, and shows the attenuated activity to promote Psl production despite that SadC300-487 is able to catalyze the synthesize of c-di-GMP in vitro and in vivo. Truncated enzyme version SadC300-487 can catalyze the synthesis of c-di-GMP in vitro, but version SadC323-487 cannot, structure-function analysis, overview Pseudomonas aeruginosa
metabolism DGC SadC is more effective than DGC WspR in promoting Psl synthesis in Pseudomonas aeruginosa strain PAO1 Pseudomonas aeruginosa
additional information the GGDEF domain of SadC has a typical GGDEF structure and the alpha-helix connecting the transmembrane domains with SadC GGDEF domain is essential for SadC to form DGC oligomers. Membrane association of SadC promotes its DGC activity by affecting the formation of active DGC oligomers. The GGDEF domain of SadC has a typical GGDEF structure Pseudomonas aeruginosa
physiological function cyclic diguanosine monophosphate (c-di-GMP) is one of the most important bacterial second messengers that controls many bacterial cellular functions including lifestyle switch between plankton and biofilm. Surface attachment defective (SadC) is a diguanylate cyclase (DGC) involved in the biosynthesis of c-di-GMP in Pseudomonas aeruginosa. SadC is reported to contribute to sensing of Psl and correspondingly increase of cellular c-di-GMP level. Involvement of Psl signalling in the SadC-mediated c-di-GMP synthesis Pseudomonas aeruginosa