Cloned (Comment) | Organism |
---|---|
- |
Pisum sativum |
His-tagged thioredoxin fusion protein expressed in Escherichia coli BL21gold strain | Pisum sativum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.048 | - |
UTP | pH 7.0, 35°C | Pisum sativum | |
0.048 | - |
UTP | D-glucose 1-phosphate, pH 7.0, 35°C | Pisum sativum | |
0.25 | - |
diphosphate | pH 7.0, 35°C | Pisum sativum | |
0.34 | - |
UDP-glucose | pH 7.0, 35°C | Pisum sativum | |
0.34 | - |
D-glucose 1-phosphate | pH 7.0, 35°C | Pisum sativum | |
0.34 | - |
UDP-D-glucose | pH 7.0, 35°C | Pisum sativum | |
0.48 | - |
D-glucuronic acid 1-phosphate | pH 7.0, 35°C | Pisum sativum | |
0.58 | - |
D-galactose 1-phosphate | pH 7.0, 35°C | Pisum sativum | |
0.96 | - |
L-arabinose 1-phosphate | pH 7.0, 35°C | Pisum sativum | |
0.96 | - |
arabinose 1-phosphate | pH 7.0, 35°C | Pisum sativum | |
1.98 | - |
D-xylose 1-phosphate | pH 7.0, 35°C | Pisum sativum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Pisum sativum | |
Mg2+ | absolute requirement for divalent cation, maximal activity in presence of Mg2+ or Mn2+. Maximal activity in presence of 2 mM Mg2+ | Pisum sativum | |
Mn2+ | 75% activity in comarison with Mg2+ | Pisum sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66040 | - |
x * 66040, calculated from sequence | Pisum sativum |
67000 | - |
x * 67000, SDS-PAGE | Pisum sativum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + a monosaccharide 1-phosphate | Pisum sativum | the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways | diphosphate + UDP-monosaccharide | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | Q5W915 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation, ion exchange chromatography (DEAE), hydrophobic interaction chromatography, gel filtration and ion exchange chromatography (DEAE), recombinant protein: immobilized metal ion affinity chromatography, splitting off the fused thioredoxin and His-tag, ion exchange chromatography (DEAE) | Pisum sativum |
recombinant | Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
sprout | - |
Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
30 | - |
UDP-L-arabinose pyrophosphorylase, pH 7.0, 35°C | Pisum sativum |
63.9 | - |
- |
Pisum sativum |
63.9 | - |
UDP-D-glucose pyrophosphorylase, pH 7.0, 35°C | Pisum sativum |
95 | - |
recombinant UDP-glucose pyrophosphorylase, pH 7.0, 35°C | Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + UDP-D-glucose | - |
Pisum sativum | UTP + D-glucose 1-phosphate | - |
r | |
UTP + a monosaccharide 1-phosphate | the enzyme catalyzes the formation of various UDP-sugars at the end of salvage pathways | Pisum sativum | diphosphate + UDP-monosaccharide | - |
? | |
UTP + arabinose 1-phosphate | 70.5% of enzyme activity with glucose 1-phosphate | Pisum sativum | diphosphate + UDP-arabinose | - |
r | |
UTP + D-galactose 1-phosphate | - |
Pisum sativum | diphosphate + UDP-galactose | - |
? | |
UTP + D-galactose 1-phosphate | 116.2% of enzyme activity with glucose 1-phosphate | Pisum sativum | diphosphate + UDP-galactose | - |
r | |
UTP + D-glucose 1-phosphate | - |
Pisum sativum | diphosphate + UDP-glucose | - |
r | |
UTP + D-glucose 1-phosphate | UTP specific | Pisum sativum | diphosphate + UDP-glucose | - |
r | |
UTP + D-glucuronic acid 1-phosphate | 71.3% of enzyme activity with glucose 1-phosphate | Pisum sativum | diphosphate + UDP-D-glucuronic acid | - |
r | |
UTP + D-glucuronic acid 1-phosphate | - |
Pisum sativum | diphosphate + UDP-glucuronic acid | - |
? | |
UTP + D-xylose 1-phosphate | - |
Pisum sativum | diphosphate + UDP-alpha-D-xylopyranose | - |
? | |
UTP + D-xylose 1-phosphate | 35.9% of enzyme activity with glucose 1-phosphate | Pisum sativum | diphosphate + UDP-xylose | - |
r | |
UTP + L-arabinose 1-phosphate | - |
Pisum sativum | diphosphate + UDP-beta-L-arabinopyranose | - |
? | |
UTP + N-acetyl-D-glucosamine 1-phosphate | 4.3% of enzyme activity with glucose 1-phosphate | Pisum sativum | diphosphate + UDP-N-acetylglucosamine | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 67000, SDS-PAGE | Pisum sativum |
? | x * 66040, calculated from sequence | Pisum sativum |
Synonyms | Comment | Organism |
---|---|---|
PsUSP | - |
Pisum sativum |
UDP-sugar pyrophosphorylase | - |
Pisum sativum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Pisum sativum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
loss of activity of more than 99% at 55°C | Pisum sativum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Pisum sativum |
7 | - |
pH 6.5-7.5, formation of UDP-glucose | Pisum sativum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | 80% loss of activity below pH 5.0 | Pisum sativum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pisum sativum | calculated | - |
5.82 |