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Literature summary for 2.7.7.50 extracted from

  • Itoh, N.; Mizumoto, K.; Kaziro, Y.
    Messenger RNA guanylyltransferase from Saccharomyces cerevisiae. I. Purification and subunit structure (1984), J. Biol. Chem., 259, 13923-13929.
    View publication on PubMed
Search for more literature for 2.7.7.50

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 x * 45000, alpha + x * 39000, beta, SDS-PAGE, probably alpha2beta2 Saccharomyces cerevisiae
45000
-
 x * 45000, alpha + x * 39000, beta, SDS-PAGE, probably alpha2beta2 Saccharomyces cerevisiae
130000
-
 gel filtration Saccharomyces cerevisiae
140000
-
 glycerol density gradient sedimentation Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
physically associated with mRNA 5'-triphosphatase activity Saccharomyces cerevisiae
to homogeneity Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00723
-
 purified enzyme Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + pp(5')RNA
-
 Saccharomyces cerevisiae G(5')ppp(5')RNA + diphosphate guanosine residue linked 5' through three phosphates to the 5' position of the terminal residue ?

Subunits

Subunits Comment Organism
oligomer x * 45000, alpha + x * 39000, beta, SDS-PAGE, probably alpha2beta2 Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Saccharomyces cerevisiae