Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. The recombinant protein proves to be insoluble and is unable to be recovered from the insoluble fraction of lysates of Escherichia coli. The reverse transcriptase is successfully expressed in a baculovirus vector although yields remained low | [Bacillus] caldolyticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Bacillus] caldolyticus | A2TD24 | - |
- |
[Bacillus] caldolyticus EA1 | A2TD24 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | [Bacillus] caldolyticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | poly(rC)*p(dG)(12-18) | [Bacillus] caldolyticus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | poly(rC)*p(dG)(12-18) | [Bacillus] caldolyticus EA1 | diphosphate + DNAn+1 | - |
? |