Any feedback?
Literature summary for 2.7.7.35 extracted from
- An adenosine 5-diphosphate ribose:orthophosphate adenylyltransferase from Euglena gracilis (1966), Biochim. Biophys. Acta, 118, 577-591.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by EDTA | Euglena gracilis |
General Stability
| General Stability | Organism |
|---|---|
| solution of lyophilized enzyme, 2 mg/ml, stable to repeated freeze-thaw cycles during 2 weeks | Euglena gracilis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | weak, ADP-ribose phosphorolysis | Euglena gracilis |  |
| AMP | kinetics | Euglena gracilis | |
| arsenate | kinetics | Euglena gracilis | |
| ATP | kinetics | Euglena gracilis | |
| D-ribose 5-phosphate | kinetics | Euglena gracilis | |
| IDP | weak, ADPribose phosphorolysis | Euglena gracilis | |
| additional information | no inhibition by adenosine, deoxyadenosine, 3'-AMP, CMP, CDP, UDP, NADH, NAD+, ADPglucose, UDPglucose, IAA, NEM; no inhibition by NADP+, EDTA, ribose | Euglena gracilis | |
| PCMB | ADP/phosphate-exchange and ADPribose phosphorolysis | Euglena gracilis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
ADP-ribose | pH 7.8, 35°C | Euglena gracilis | |
| 0.5 | - |
phosphate | pH 7.8, 35°C | Euglena gracilis | |
| 0.6 | - |
ADP | ADP/phosphate exchange | Euglena gracilis | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Euglena gracilis | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no Mn2+ or Mg2+ requirement | Euglena gracilis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphate + ADP-ribose | Euglena gracilis | - |
ADP + D-ribose 5-phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Euglena gracilis | - |
var. bacillaris Pringsheim | - |
| no activity in Chlorella pyrenoidosa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Euglena gracilis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | heterotrophically or autotrophically grown cells | Euglena gracilis | - |
| cell culture | UV-mutant cells incapable of forming chloroplasts | Euglena gracilis | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.51 | - |
partially purified enzyme | Euglena gracilis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, undialyzed purified enzyme, lyophilized and placed under vacuum, 35% loss of activity within 2 months | Euglena gracilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with CDP, GDP, UDP, ADP-glucose and UDP-glucose | Euglena gracilis | ? | - |
? | |
| additional information | no activity with AMP and ATP | Euglena gracilis | ? | - |
? | |
| phosphate + ADP | ADP/phosphate-exchange reaction, inorganic phosphate is incorporated into terminal position of ADP | Euglena gracilis | ADP + phosphate | - |
r | |
| phosphate + ADP | IDP can replace ADP with 16% efficiency | Euglena gracilis | ADP + phosphate | - |
r | |
| phosphate + ADP-ribose | - |
Euglena gracilis | ADP + D-ribose 5-phosphate | - |
ir | |
| phosphate + ADP-ribose | highly specific | Euglena gracilis | ADP + D-ribose 5-phosphate | - |
ir | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Euglena gracilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 29 | 56 | about half-maximal activity at 29°C and 56°C | Euglena gracilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
3 min, inactivation | Euglena gracilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
ADP/phosphate-exchange | Euglena gracilis |
| 8 | - |
ADP-ribose phosphorolysis | Euglena gracilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | 8.5 | ADP/phosphate-exchange, about 60% of maximal activity at pH 6.7 and about half-maximal activity at pH 8.5 | Euglena gracilis |
| 6.8 | 9.4 | about half-maximal activity at pH 6.8 and pH 9.4 | Euglena gracilis |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
D-ribose 5-phosphate | phosphorolysis | Euglena gracilis | |
| 0.09 | - |
D-ribose 5-phosphate | pH 7.8, 35°C | Euglena gracilis | |
| 0.11 | - |
D-ribose 5-phosphate | pH 7.8, 35°C | Euglena gracilis | |
| 0.11 | - |
D-ribose 5-phosphate | ADP/phosphate-exchange reaction | Euglena gracilis | |
| 0.41 | - |
arsenate | pH 7.8, 35°C | Euglena gracilis | |
| 0.41 | - |
arsenate | ADP/phosphate-exchange reaction | Euglena gracilis | |
| 0.57 | - |
arsenate | phosphorolysis | Euglena gracilis | |
| 0.57 | - |
arsenate | pH 7.8, 35°C | Euglena gracilis | |
| 1 | - |
AMP | phosphorolysis | Euglena gracilis | |
| 1 | - |
AMP | pH 7.8, 35°C | Euglena gracilis | |
| 1.3 | - |
ATP | pH 7.8, 35°C | Euglena gracilis | |
| 1.3 | - |
ATP | ADP/phosphate-exchange reaction | Euglena gracilis | |
| 1.5 | - |
AMP | pH 7.8, 35°C | Euglena gracilis | |
| 1.5 | - |
AMP | ADP/phosphate-exchange reaction | Euglena gracilis | |
| 2.9 | - |
ATP | phosphorolysis | Euglena gracilis | |
| 2.9 | - |
ATP | pH 7.8, 35°C | Euglena gracilis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
