Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli ER2566 cells | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
apo form and in complex with ATP, counter-diffusion method, using 40 mM cacodylate buffer pH 5.5 containing 10 mM MgCl2, 0.15 mM NaCl, 20 mM cobalt hexamine and 15% (w/v) 2-methyl-2,4-pentanediol as precipitant solution | Mycobacterium tuberculosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WPA5 | - |
- |
Mycobacterium tuberculosis H37Rv | P9WPA5 | - |
- |
Purification (Comment) | Organism |
---|---|
Q Sepharose XL resin column chromatography, Q Sepharose HP resin column chromatography, and Sepharose S-200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pantetheine 4'-phosphate | - |
Mycobacterium tuberculosis | diphosphate + 3'-dephospho-CoA | - |
r | |
ATP + pantetheine 4'-phosphate | - |
Mycobacterium tuberculosis H37Rv | diphosphate + 3'-dephospho-CoA | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | x-ray crystallography | Mycobacterium tuberculosis |
Synonyms | Comment | Organism |
---|---|---|
phosphopantetheine adenylyltransferase | - |
Mycobacterium tuberculosis |
PPAT | - |
Mycobacterium tuberculosis |