Literature summary for 2.7.7.27 extracted from
Gomez Casati, D.F.; Aon, M.A.; Iglesias, A.A.
Kinetic and structural analysis of the ultrasensitive behaviour of cyanobacterial ADP-glucose pyrophosphorylase (2000), Biochem. J., 350, 139-147.
No PubMed abstract available
Activating Compound
Activating Compound |
Comment |
Organism |
Structure |
3-phosphoglycerate |
the polyethylene glycerol induced molecular crowding increases the sensitivity of enzyme to the cross-talk between 3-phosphoglycerate and phosphate, the ultrasensitive behaviour is correlated with intramolecular conformational changes induced in the tertiary structure of the homotetrameric enzyme |
Anabaena sp. |
|
Cloned(Commentary)
Cloned (Comment) |
Organism |
expression in Escherichia coli AC70R1-504 |
Anabaena sp. |
Inhibitors
Inhibitors |
Comment |
Organism |
Structure |
phosphate |
the inhibitory action of phosphate interferes with the activating role exerted by 3-phosphoglycerate, a 2fold higher positive cooperation is induced by allosteric inhibitor in crowded medium in comparison to the aqueous condition |
Anabaena sp. |
|
KM Value [mM]
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
additional information |
- |
additional information |
comparison of Km of alpha-D-glucose 1-phosphate at different ATP concentrations, at two levels of 3-phosphoglycerate and in aqueous or by polyethylenglycol crowded medium, comparison of Km of ATP at different alpha-D-glucose 1-phosphate concentrations, at two levels of 3-phosphoglycerate and in aqueous or by polyethylenglycol crowded medium |
Anabaena sp. |
|
Molecular Weight [Da]
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
200000 |
- |
gel filtration |
Anabaena sp. |
Organism
Organism |
UniProt |
Comment |
Textmining |
Anabaena sp. |
- |
PCC 7120, cyanobacterium |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
recombinant enzyme |
Anabaena sp. |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
ATP + alpha-D-glucose 1-phosphate |
- |
Anabaena sp. |
diphosphate + ADP-glucose |
- |
? |
|