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Literature summary for 2.7.7.24 extracted from
- One-pot four-enzyme synthesis of thymidine diphosphate-l-rhamnose (2016), Chem. Commun. (Camb.), 52, 13995-13998 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Streptococcus pneumoniae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | strong inhibition at 10 mM | Streptococcus pneumoniae |  |
| Cu2+ | strong inhibition at 10 mM | Streptococcus pneumoniae | |
| Fe2+ | strong inhibition at 10 mM | Streptococcus pneumoniae | |
| K+ | complete inhibition at 10 mM | Streptococcus pneumoniae | |
| Mn2+ | strong inhibition at 10 mM | Streptococcus pneumoniae | |
| Na+ | complete inhibition at 10 mM | Streptococcus pneumoniae | |
| Ni2+ | complete inhibition at 10 mM | Streptococcus pneumoniae | |
| Zn2+ | complete inhibition at 10 mM | Streptococcus pneumoniae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required for activity, highest activity between 0.63 and 40 mM, 5-10 mM used in assay conditions | Streptococcus pneumoniae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTTP + alpha-D-glucose 1-phosphate | Streptococcus pneumoniae | - |
diphosphate + dTDP-alpha-D-glucose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus pneumoniae | O54574 | serotype 23F | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni affinity column chromatography | Streptococcus pneumoniae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dTTP + alpha-D-glucose 1-phosphate | - |
Streptococcus pneumoniae | diphosphate + dTDP-alpha-D-glucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 35000, SDS-PAGE | Streptococcus pneumoniae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Cps23FL | - |
Streptococcus pneumoniae |
| Glc-1-P thymidylyltransferase | - |
Streptococcus pneumoniae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Streptococcus pneumoniae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Streptococcus pneumoniae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | more than 80% activity between pH 7.0 and 8.0 | Streptococcus pneumoniae |
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Release 2023.1 (January 2023)
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