Cloned (Comment) | Organism |
---|---|
native and mutant enzymes expressed as His-tag fusion protein in Escherichia coli M15 | Giardia intestinalis |
Protein Variants | Comment | Organism |
---|---|---|
G108A | dramatically reduced activity, significant increase in melting temperature | Giardia intestinalis |
G210A | dramatically reduced activity, significant decrease in melting temperature | Giardia intestinalis |
General Stability | Organism |
---|---|
substrate binding increases enzyme stability against thermal inactivation | Giardia intestinalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
N-acetyl-alpha-D-glucosamine 1-phosphate | purified recombinant native enzyme, pH 7.5, 37°C | Giardia intestinalis | |
0.038 | - |
UTP | Mg-UTP, purified recombinant native enzyme, pH 7.5, 37°C | Giardia intestinalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | in complex with UTP | Giardia intestinalis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
gel filtration | Giardia intestinalis |
49624 | - |
1 * 50000, SDS-PAGE, 1 * 49624, calculated from the deduced amino acid sequence, native mass by gel filtration | Giardia intestinalis |
50000 | - |
1 * 50000, SDS-PAGE, 1 * 49624, calculated from the deduced amino acid sequence, native mass by gel filtration | Giardia intestinalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | Giardia intestinalis | involved in the synthesis of UDP-N-acetyl-galactoseamine, which is important for cyst wall synthesis | diphosphate + UDP-N-acetyl-D-glucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Giardia intestinalis | Q8MUP8 | Portland 1 strain, ATCC30888 | - |
Purification (Comment) | Organism |
---|---|
recombinant proteins using His-tag | Giardia intestinalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
72 | - |
purified recombinant native enzyme, pH 7.5, 37°C | Giardia intestinalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | - |
Giardia intestinalis | diphosphate + UDP-N-acetyl-D-glucosamine | - |
? | |
UTP + N-acetyl-alpha-D-glucosamine 1-phosphate | involved in the synthesis of UDP-N-acetyl-galactoseamine, which is important for cyst wall synthesis | Giardia intestinalis | diphosphate + UDP-N-acetyl-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50000, SDS-PAGE, 1 * 49624, calculated from the deduced amino acid sequence, native mass by gel filtration | Giardia intestinalis |