Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli strain Rosetta(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
FMN | 70% inhibited when using FMN concentrations higher than 0.004 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
FMN | at 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | strict requirement for MgCl2 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54190 | - |
calculated from amino acid sequence | Homo sapiens |
60000 | - |
SDS-PAGE, isoform 2 | Homo sapiens |
63000 | - |
SDS-PAGE | Homo sapiens |
65270 | - |
calculated from amino acid sequence | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q8NFF5 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-chelating Sepharose Fast Flow column chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.59 | - |
crude cell extract, at 37°C | Homo sapiens |
6.8 | - |
after 12.5fold purification, at 37°C | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + FMN | - |
Homo sapiens | diphosphate + FAD | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAD synthetase | - |
Homo sapiens |
FADS1 | - |
Homo sapiens |
FADS2 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |