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Literature summary for 2.7.7.15 extracted from

  • Helmink, B.A.; Braker, J.D.; Kent, C.; Friesen, J.A.
    Identification of lysine 122 and arginine 196 as important functional residues of rat CTP:phosphocholine cytidylyltransferase a (2003), Biochemistry, 42, 5043-5051.
    View publication on PubMed
Search for more literature for 2.7.7.15

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
K122A decreased Vmax Rattus norvegicus
K122R decreased Vmax Rattus norvegicus
R196K decreased Vmax, 5fold increased Km for phosphorylcholine, 23fold increased Km for CTP Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.45
-
 phosphocholine wild-type, pH 7, 37°C Rattus norvegicus
1.54
-
 CTP wild-type, pH 7, 37°C Rattus norvegicus
2.09
-
 CTP K122A mutant, pH 7, 37°C Rattus norvegicus
2.35
-
 phosphocholine R196K mutant, pH 7, 37°C Rattus norvegicus
4.78
-
 CTP K122R mutant, pH 7, 37°C Rattus norvegicus
35.7
-
 CTP R196K mutant, pH 7, 37°C Rattus norvegicus
36
-
 phosphocholine K122R mutant, pH 7, 37°C Rattus norvegicus
212
-
 phosphocholine K122A mutant, pH 7, 37°C Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
CTP + phosphocholine = diphosphate + CDP-choline catalytic mechanism Rattus norvegicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CTP + choline phosphate
-
 Rattus norvegicus diphosphate + CDPcholine
-
 ?