Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Helicobacter pylori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
alpha-D-mannose 1-phosphate | pH 8.0, 37°C, cosubstrate GTP | Helicobacter pylori | |
0.1 | - |
GDPmannose | pH 8.0, 37°C, cosubstrate diphosphate | Helicobacter pylori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Helicobacter pylori | |
Mn2+ | 30% of activity with Mg2+ | Helicobacter pylori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
x * 55000, SDS-PAGE | Helicobacter pylori |
55303 | - |
x * 55303, deduced from nucleotide sequence | Helicobacter pylori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | Helicobacter pylori | enzyme regulates GDP-D-mannose synthesis through feedback inhibition | GDPmannose + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helicobacter pylori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, Ni-nitrilotriacetic acid column, Superdex 200 | Helicobacter pylori |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose | bifunctional enzyme with both phosphomannose isomerase and GDP-D-mannose pyrophosphorylase activity | Helicobacter pylori |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GTP + alpha-D-mannose 1-phosphate | - |
Helicobacter pylori | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | enzyme regulates GDP-D-mannose synthesis through feedback inhibition | Helicobacter pylori | GDPmannose + diphosphate | - |
r | |
GTP + alpha-D-mannose 1-phosphate | - |
Helicobacter pylori | diphosphate + GDP-mannose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Helicobacter pylori |
? | x * 55303, deduced from nucleotide sequence | Helicobacter pylori |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10.3 | - |
alpha-D-mannose 1-phosphate | pH 8.0, 37°C, cosubstrate GTP | Helicobacter pylori | |
22.1 | - |
GDPmannose | pH 8.0, 37°C, cosubstrate diphosphate | Helicobacter pylori |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
GDP-mannose synthesis, forward reaction is very sensitive to pH fluctuation | Helicobacter pylori |
7 | 9 | GTP synthesis | Helicobacter pylori |