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Literature summary for 2.7.6.2 extracted from
- Thiamin pyrophosphokinase is required for thiamin cofactor activation in Arabidopsis (2007), Plant Mol. Biol., 65, 151-162.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| isozymes TPK1 and TPK2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21, expression of fluorescent tagged isozymes in cytosol of mesophyll protoplasts | Arabidopsis thaliana |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of a double knockout mutant lacking isozymes TPK1 and TPK2, the mutant shows severely depressed levels of TPP and elevated levels of free thiamine compared to the wild-type enzyme | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00096 | - |
thiamine | AtTPK1 | Arabidopsis thaliana |  |
| 0.00096 | - |
thiamine | pH 8.0, 37°C, isozyme TPK1 | Arabidopsis thaliana | |
| 0.00128 | - |
thiamine | AtTPK2 | Arabidopsis thaliana | |
| 0.00128 | - |
thiamine | pH 8.0, 37°C, isozyme TPK2 | Arabidopsis thaliana | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Arabidopsis thaliana | 5829 | - |
| cytosol | isozymes TPK1 and TPK2 | Arabidopsis thaliana | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + thiamine | Arabidopsis thaliana | thiamine pyrophosphokinase is required for thiamine cofactor activation in Arabidopsis | AMP + thiamine diphosphate | - |
? | |
| thiamin + ATP | Arabidopsis thaliana | - |
thiamindiphosphate + AMP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Arabidopsis thaliana | - |
isozymes TPK1 and TPK2 | - |
| Arabidopsis thaliana | F4IV16 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | - |
Arabidopsis thaliana | - |
| leaf | - |
Arabidopsis thaliana | - |
| additional information | isozymes TPK1 and TPK2 are biochemically redundant cytosolic proteins that are similarly expressed throughout different plant tissues | Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| silique | - |
Arabidopsis thaliana | - |
| stem | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + thiamine | thiamine pyrophosphokinase is required for thiamine cofactor activation in Arabidopsis | Arabidopsis thaliana | AMP + thiamine diphosphate | - |
? | |
| ATP + thiamine | no TMP kinase activity is shown by either isozymes TPK1 or TPK2 | Arabidopsis thaliana | AMP + thiamine diphosphate | - |
? | |
| thiamin + ATP | - |
Arabidopsis thaliana | thiamindiphosphate + AMP | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| At1g02880 (AtTPK1) | - |
Arabidopsis thaliana |
| At2g44750 (AtTPK2) | - |
Arabidopsis thaliana |
| thiamin pyrophosphokinase | - |
Arabidopsis thaliana |
| TPK | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
isozyme TPK2 | Arabidopsis thaliana |
| 37 | - |
AtTPK2 | Arabidopsis thaliana |
| 45 | - |
AtTPK1 | Arabidopsis thaliana |
| 45 | - |
isozyme TPK1 | Arabidopsis thaliana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 50 | 37°C: optimal activity of TPK2, 50°C: TPK2 is completely inactive | Arabidopsis thaliana |
| 45 | 55 | TPK1 | Arabidopsis thaliana |
| 50 | - |
AtTPK2 is completely inactive | Arabidopsis thaliana |
| 55 | - |
AtTPK1 is still active | Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
inactivation of isozyme TPK2 | Arabidopsis thaliana |
| 55 | - |
isozyme TPK1 shows remaining activity | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Arabidopsis thaliana |
| 7 | - |
isozymes TPK1 or TPK2 | Arabidopsis thaliana |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | - |
Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Arabidopsis thaliana | |
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