Literature summary for 2.7.6.1 extracted from

Esipov, R.; Abramchik, Y.; Fateev, I.; Muravyova, T.; Artemova, K.; Konstantinova, I.; Kuranova, I.; Miroshnikov, A.
Recombinant phosphoribosyl pyrophosphate synthetases from Thermus thermophilus HB27 Isolation and properties (2016), Russ. J. Bioorg. Chem., 42, 512-521 .

No PubMed abstract available

Search for more literature for 2.7.6.1

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.011	-	D-ribose 5-phosphate	pH 8.0, 75°C	Thermus thermophilus
0.013	-	ATP	pH 8.0, 75°C	Thermus thermophilus
0.034	-	D-ribose 5-phosphate	pH 8.0, 75°C	Thermus thermophilus
0.038	-	ATP	pH 8.0, 75°C	Thermus thermophilus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
150000	-	gel filtration	Thermus thermophilus
158000	-	gel filtration	Thermus thermophilus

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	Q72I58	isoform PRPPS2	-
Thermus thermophilus	Q72IE8	isoform PRPPS1	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + D-ribose 5-phosphate	-	Thermus thermophilus	AMP + 5-phospho-alpha-D-ribose 1-diphosphate	-	?

Subunits

Subunits	Comment	Organism
hexamer	6 * 34300, SDS-PAGE	Thermus thermophilus
hexamer	6 * 34600, SDS-PAGE	Thermus thermophilus

Synonyms

Synonyms	Comment	Organism
PRPPS1	-	Thermus thermophilus
PRPPS2	-	Thermus thermophilus
TT_C1184	-	Thermus thermophilus
TT_C1274	-	Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.43	-	D-ribose 5-phosphate	pH 8.0, 75°C	Thermus thermophilus
0.53	-	ATP	pH 8.0, 75°C	Thermus thermophilus
12	-	ATP	pH 8.0, 75°C	Thermus thermophilus
13	-	D-ribose 5-phosphate	pH 8.0, 75°C	Thermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
14	-	ATP	pH 8.0, 75°C	Thermus thermophilus
39	-	D-ribose 5-phosphate	pH 8.0, 75°C	Thermus thermophilus
390	-	D-ribose 5-phosphate	pH 8.0, 75°C	Thermus thermophilus
960	-	ATP	pH 8.0, 75°C	Thermus thermophilus

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Release 2023.1 (January 2023)