Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | activates, maximal activity at 190 mM phosphate | Methanocaldococcus jannaschii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion technique | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | a single ADP binding site, the active site, is present per subunit | Methanocaldococcus jannaschii | |
alpha,beta-methylene ATP | - |
Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.6 | - |
ATP | pH and temperature not specified in the publication | Methanocaldococcus jannaschii | |
2.8 | - |
D-ribose 5-phosphate | pH and temperature not specified in the publication | Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | the activity of the enzyme depends on the presence of divalent cations, of which Mg2+ is the most effective. Mn2+ is also accepted, but the activity is less than 15% of that obtained with Mg2+, when assayed at pH 9.5 | Methanocaldococcus jannaschii | |
Mn2+ | the activity of the enzyme depends on the presence of divalent cations, of which Mg2+ is the most effective. Mn2+ is also accepted, but the activity is less than 15% of that obtained with Mg2+, when assayed at pH 9.5 | Methanocaldococcus jannaschii | |
additional information | the enzyme is essentially inactive in the presence of Cu2+, Ca2+, Co2+, Ni2+ or Fe2+ | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32229 | - |
4 * 32229, calculated from sequence | Methanocaldococcus jannaschii |
147000 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58761 | - |
- |
Methanocaldococcus jannaschii DSM 2661 | Q58761 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methanocaldococcus jannaschii |
Storage Stability | Organism |
---|---|
4°C or -20°C in 25 mM TrisHCl (pH 7.6), 50% (v/v) glycerol, purified enzyme is stable for at least 90 days when stored at | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | the enzyme accepts ATP and dATP equally well as diphosphoryl donor | Methanocaldococcus jannaschii | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
ATP + D-ribose 5-phosphate | the enzyme accepts ATP and dATP equally well as diphosphoryl donor | Methanocaldococcus jannaschii DSM 2661 | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
dATP + D-ribose 5-phosphate | the enzyme accepts ATP and dATP equally well as diphosphoryl donor | Methanocaldococcus jannaschii | dAMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
dATP + D-ribose 5-phosphate | the enzyme accepts ATP and dATP equally well as diphosphoryl donor | Methanocaldococcus jannaschii DSM 2661 | dAMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
? | |
additional information | the enzyme is essentially unable to use GTP (0.4% of the activity obtained with ATP), UTP (0.5%) or CTP (3%) as phosphoryl donor | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | the enzyme is essentially unable to use GTP (0.4% of the activity obtained with ATP), UTP (0.5%) or CTP (3%) as phosphoryl donor | Methanocaldococcus jannaschii DSM 2661 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32229, calculated from sequence | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
phosphoribosyl diphosphate synthase | - |
Methanocaldococcus jannaschii |
PRPP synthase | - |
Methanocaldococcus jannaschii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Methanocaldococcus jannaschii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
72 | 95 | 70% of maximal activity at 72°C and 95°C | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
at pH 9.0, half-life: 8 min | Methanocaldococcus jannaschii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9.5 | - |
- |
Methanocaldococcus jannaschii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
10 | - |
the activity profile declines steeply above pH 10. At least in part, this reduction in activity at high pH values may be caused by the formation of a magnesium phosphate precipitate, and consequently cause Mg2+ depletion. At lower pH the activity increases more or less linearly from pH 6.0 to pH 9.5 | Methanocaldococcus jannaschii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
ADP | pH and temperature not specified in the publication | Methanocaldococcus jannaschii | |
0.85 | - |
alpha,beta-methylene ATP | pH and temperature not specified in the publication | Methanocaldococcus jannaschii |