Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
purified enzyme appears to exist as complex aggregates composed of heterogeneous components | Rattus norvegicus |
34000 | - |
x * 34000 + x * 38000 + x * 40000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit | Rattus norvegicus |
38000 | - |
x * 34000 + x * 38000 + x * 40000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit | Rattus norvegicus |
40000 | - |
x * 34000 + x * 38000 + x * 40000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit | Rattus norvegicus |
1000000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | Rattus norvegicus | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
polyethylene glycol precipitation, hydroxyapatite fractionation, gel filtration on Toyopearl HW-65F, chromatography on DEAE-Toyopearl 650S and gel filtration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.28 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80ºC, potassium phosphate buffer, pH 7.4, stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + D-ribose 5-phosphate | - |
Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + D-ribose 5-phosphate | the product phosphoribosyldiphosphate is required for the biosynthesis of purine, pyrimidine and pyridine nucleotides, L-histidine and L-tryptophan | Rattus norvegicus | AMP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000 + x * 38000 + x * 40000, SDS-PAGE, the 34000 Da subunit is the catalytic subunit | Rattus norvegicus |