Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cGMP | lower affinity or extent of activation than GTP | Escherichia coli | |
dGTP | lower affinity or extent of activation than GTP | Escherichia coli | |
dialdehyde-GTP | slight stimulatory effect, 30% maximal increase in UMP-kinase activity | Escherichia coli | |
GMP | lower affinity or extent of activation than GTP | Escherichia coli | |
GTP | activates only the wild-type, not the mutant D201N | Escherichia coli | |
guanosine | lower affinity or extent of activation than GTP | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
overproduced in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D201N | exhibits 10% of the wild-type activity, has altered stability and regulatory properties | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | mutant D201N | Escherichia coli | |
guanylyl imidodiphosphate | mutant D201N | Escherichia coli | |
additional information | no inhibition by dUMP, CMP, dCMP or TMP | Escherichia coli | |
UTP | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
UMP | at 1 mM ATP, wild-type | Escherichia coli | |
0.11 | - |
UMP | mutant D201N | Escherichia coli | |
0.12 | - |
ATP | at 1 mM UMP, wild-type | Escherichia coli | |
0.32 | - |
ATP | mutant D201N | Escherichia coli | |
1 | - |
GTP | wild-type | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25800 | - |
monomer, calculated from amino acid sequence | Escherichia coli |
156000 | - |
sedimentation equilibrium | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | Escherichia coli | - |
ADP + UDP | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A7E9 | - |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.13 | - |
mutant D201N, rate of the reverse reaction | Escherichia coli |
3.6 | - |
mutant D201N, in the presence of 1 mM ATP and 1 mM UMP | Escherichia coli |
160 | - |
wild-type, in the presence of ATP and GTP | Escherichia coli |
Storage Stability | Organism |
---|---|
room temperature, 0.1 M borate buffer (pH 8.5), 2 months, no loss of activity | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + UMP | - |
Escherichia coli | ADP + UDP | - |
r | |
ATP + UMP | 30°C, pH 7.4, 2 mM MgCl2 | Escherichia coli | ADP + UDP | - |
r | |
GTP + UMP | reaction rate is 3-5% of that with ATP | Escherichia coli | GDP + UDP | - |
r | |
additional information | no reaction with dUMP, CMP, dCMP or TMP as substrate | Escherichia coli | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha6, sedimentation equilibrium | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
UMP-kinase | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
51 | - |
mutant D201N is half-inactivated | Escherichia coli |
64 | - |
wild-type protein is half-inactivated | Escherichia coli |