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Literature summary for 2.7.4.2 extracted from

  • Zheng, H.; Yu, M.; Pu, C.; Chen, M.; Li, F.; Shen, Y.; Huang, L.
    Cloning and expression analysis of 5-phosphomevalonate kinase gene (CcPMK) in Cinnamomum camphora (2020), Zhongguo Zhong Yao Za Zhi, 45, 78-84 .
    View publication on PubMed
Search for more literature for 2.7.4.2

Cloned(Commentary)

Cloned (Comment) Organism
gene CcPMK, DNA and amino acid sequence determination and analysis, cDNA on the basis of the transciptome database of Cinnamomum camphora, sequence comparison and phylogenetic analysis Cinnamomum camphora

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information sequence analysis reveals no signal peptide and transmembrane structure in the putative protein Cinnamomum camphora
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-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Cinnamomum camphora

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-5-phosphomevalonate Cinnamomum camphora
-
 ADP + (R)-5-diphosphomevalonate
-
 r

Organism

Organism UniProt Comment Textmining
Cinnamomum camphora A0A6G6C941
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-

Source Tissue

Source Tissue Comment Organism Textmining
branch low expression level Cinnamomum camphora
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additional information results of real-time PCR indicate that the expression level of CcPMK in borneol type is higher than that in linalool type, cineol type, iso-nerolidol type, and camphor type variants of Cinnamomum camphora trees. CcPMK expressed highest in roots and lowest in branches Cinnamomum camphora
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root high expression level Cinnamomum camphora
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-5-phosphomevalonate
-
 Cinnamomum camphora ADP + (R)-5-diphosphomevalonate
-
 r

Subunits

Subunits Comment Organism
? x * 56140, sequence calculation Cinnamomum camphora
More Cinnamomum camphora PMK has 3 PMK protein family motifs and 1 ATP binding site Gly-Leu-Gly-Ser-Ser-Ala-Ala, and its 3D structure contains a catalytic pocket structure, proving CcPMK as a member of PMK gene family Cinnamomum camphora

Synonyms

Synonyms Comment Organism
CcPMK
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 Cinnamomum camphora
PMK
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 Cinnamomum camphora

pI Value

Organism Comment pI Value Maximum pI Value
Cinnamomum camphora sequence calculation
-
 7.64

General Information

General Information Comment Organism
evolution Cinnamomum camphora PMK has 3 PMK protein family motifs and 1 ATP binding site Gly-Leu-Gly-Ser-Ser-Ala-Ala, and its 3 D structure contains a catalytic pocket structure, proving CcPMK as a member of PMK gene family Cinnamomum camphora
physiological function the 5-phosphomevalonate kinase(PMK) is a key enzyme in mevalonate(MVA) pathway which reversibly catalyzes the phosphorylation of mevalonate 5-phosphate(MVAP) to form mevalonate-5-diphosphate(MVAPP) in the presence of ATP and divalent metal ion such as Mg2+. The expression level of CcPMK was different among five chemical types and different plant tissues Cinnamomum camphora