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Literature summary for 2.7.4.2 extracted from

  • Motoyama, K.; Sobue, F.; Kawaide, H.; Yoshimura, T.; Hemmi, H.
    Conversion of mevalonate 3-kinase into 5-phosphomevalonate 3-kinase by single amino acid mutations (2019), Appl. Environ. Microbiol., 85, e00256-19 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.4.2

Cloned(Commentary)

Cloned (Comment) Organism
gene ERG8, the pBAD plasmid containing the genes of MVK, PMK, and DMD from Saccharomyces cerevisiae (pBAD-ScMPD) is used for expression of a part of the classical MVA pathway (Fig. 6B). The codon usage of the MVK and PMK genes has been optimized for Escherichia coli, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + (R)-5-phosphomevalonate Saccharomyces cerevisiae
-
 ADP + (R)-5-diphosphomevalonate
-
 ?
ATP + (R)-5-phosphomevalonate Saccharomyces cerevisiae ATCC 204508
-
 ADP + (R)-5-diphosphomevalonate
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P24521
-
-
Saccharomyces cerevisiae ATCC 204508 P24521
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + (R)-5-phosphomevalonate
-
 Saccharomyces cerevisiae ADP + (R)-5-diphosphomevalonate
-
 ?
ATP + (R)-5-phosphomevalonate
-
 Saccharomyces cerevisiae ATCC 204508 ADP + (R)-5-diphosphomevalonate
-
 ?

Synonyms

Synonyms Comment Organism
ERG8
-
 Saccharomyces cerevisiae
PMK
-
 Saccharomyces cerevisiae

General Information

General Information Comment Organism
metabolism almost all eukaryotes, most of the MVA pathway-utilizing bacteria, and the archaea of the order Sulfolobales utilize mevalonate kinase (MVK), phosphomevalonate kinase (PMK), and diphosphomevalonate decarboxylase (DMD) for that purpose. The pathway that includes this set of enzymes is called the classical MVA pathway because it was discovered more than half a century ago Saccharomyces cerevisiae
physiological function mevalonate 3-kinase catalyzes the ATP-dependent 3-phosphorylation of mevalonate but does not catalyze the subsequent decarboxylation as related decarboxylases do Saccharomyces cerevisiae